Description
T4 UvsX Recombinase, derived from the T4 bacteriophage, is a homolog of the RecA/Rad51 family and plays a critical role in the repair of double-strand DNA breaks and the restart of replication forks. This recombinase collaborates with other DNA-binding proteins or cofactors to form a nucleoprotein complex with single-stranded DNA (ssDNA). The complex identifies complementary regions on target DNA for hybridization, facilitating subsequent strand displacement reactions. The enzyme exhibits no nuclease activity.
Product Components
|
NO. |
Name |
11079ES60 (100 μg) |
11079ES03 (1 mg) |
11079ES08 (5 mg) |
11079ES20 (20 mg) |
|
11079 |
T4 UvsX protein (2 μg/μL) |
50 μL |
500 μL |
2.5 mL |
10 mL |
Product Applications
Primarily used in isothermal amplification techniques, such as Recombinase Polymerase Amplification (RPA).
Enzyme Storage Buffer Composition
50 mM Tris-HCl, 50 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 20% Glycerol, pH 7.5 at 25°C.
Shipping and Storage Conditions
Shipped with ice packs; store at -20°C. Shelf life: 2 years. Avoid repeated freeze-thaw cycles.
Precautions
1. The glycerol content in the T4 UvsX Recombinase storage buffer is 20%. It is recommended to aliquot the enzyme into separate portions to avoid repeated freeze-thaw cycles.
2. This product is intended for research use only.
3. For your safety and health, please wear a lab coat and disposable gloves during handling.
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The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
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