Description
Product description
Hifair™ AdvanceFast 1st Strand cDNA Synthesis SuperMix for qPCR (gDNA digester plus) is an upgraded version of Hifair™ Ⅲ 1st Strand cDNA Synthesis SuperMix for qPCR (gDNA digester plus). Compared to the previous generation Hifair™ Ⅲ Reverse Transcriptase, this product exhibits enhanced tolerance to inhibitors, faster reverse transcription speed, and higher cDNA yield, making it suitable for reverse transcription reactions of RNA from animals, plants, and microorganisms. The gDNA Digester Mix -V2 included in this product has been significantly improved in its ability to remove residual DNA, effectively digesting up to 1000 ng of DNA, ensuring more reliable results by eliminating DNA contamination in RNA templates.
Hifair™ AdvanceFast SuperMix contains all components required for reverse transcription. Simply add RNA template and RNase-free H2O to initiate the reaction, while simultaneously terminating the activity of gDNA Digester to ensure cDNA integrity.
The reverse transcription products are suitable for dye-based and probe-based qPCR. However, they are not recommended for long-fragment amplification such as gene cloning. For such applications, Hifair™ AdvanceFast 1st Strand cDNA Synthesis Kit (Cat#11150ES) is recommended for high-efficiency reverse transcription.
Specifications
|
Cat.No. |
11155ES10 / 11155ES60 |
|
Size |
10 T / 100 T |
Components
|
Components No. |
Name |
11155ES10 |
11155ES60 |
|
11155-A |
Hifair™ AdvanceFast SuperMix |
60 μL |
600 μL |
|
11155-B |
gDNA Digester Mix -V2 |
20 μL |
200 μL |
|
11155-C |
RNase Free H2O |
200 μL |
2×1 mL |
Storage
This product should be stored at -25~-15℃ for 1 years.
Instructions
1. When DNA Removal is Required
1）DNA Digestion
Prepare the following mixture in an RNase-free microcentrifuge tube, mix gently by pipetting, and incubate at 37℃ for 2 min:
|
Components |
Volume（μL） |
|
gDNA Digester Mix -V2 |
2 |
|
Total RNA |
10 pg–2 µg* |
|
or mRNA |
10 pg–500 ng |
|
RNase Free H2O |
Up to 14 |
*It is recommended to use ≤ 2 µg of Total RNA. If the target gene expression is low, the amount can be increased up to 5 µg of Total RNA.
2）Reverse Transcription Reaction Setup (20 µL System):
|
Components |
Volume（μL） |
|
Reaction mixture from step 1 |
14 |
|
Hifair™ AdvanceFast SuperMix |
6 |
3）Reaction Program:
|
Temperature |
Time |
|
50℃ |
5 min |
|
85℃ |
5 sec |
2. When DNA Removal is Not Required
1）Reverse Transcription Reaction Setup (20 µL System):
|
Components |
Volume（μL） |
|
Hifair™ AdvanceFast SuperMix |
6 |
|
Total RNA |
10 pg–2 µg* |
|
or mRNA |
10 pg–500 ng |
|
RNase Free H2O |
Up to 20 |
*It is recommended to use ≤ 2 µg of Total RNA. If the target gene expression is low, the amount can be increased up to 5 µg of Total RNA.
2）Reaction Program:
|
Temperature |
Time |
|
50℃ |
5 min |
|
85℃ |
5 sec |
Notes
1. This product is for research use only.
2. Please operate with lab coats and disposable gloves，for your safety.
3. Perform all pipetting and mixing steps on ice to maintain sample integrity.
4. Gently invert and centrifuge each component at low speed before use to ensure proper mixing.
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FAQ
The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
Certain applications may require additional third-party intellectual property rights.
Yeasen is dedicated to ethical science, believing our research should address critical questions while ensuring safety and ethical standards.