Description
The product is a premixed solution for 2× real-time quantitative PCR amplification by the probe method. The antibody hot-start UNICONTM DNA Polymerase used can effectively inhibit the amplification caused by the non-specific annealing of primers. At the same time, the formula optimizes the factors that can effectively inhibit non-specific PCR amplification and improve the amplification efficiency of PCR reaction, which is suitable for the amplification of low-concentration templates, so that quantitative PCR can obtain a good standard curve in a wide quantitative area.
Features
- High specificity
- High sensitivity
Applications
- Gene Expression
Specifications
| Hot Start | Built-in hot start |
| Detection method | Primer-probe detection |
| PCR method | RT-qPCR |
| Polymerase | Taq DNA polymerase |
| Type of sample | RNA |
| Application equipment | Applied Biosystems: 5700, 7000, 7300, 7700, 7900HT Fast, StepOne™, StepOne™ Plus; 7500, 7500 Fast, ViiA™7, QuantStudio™ 3 and 5, QuantStudio™ 6, 7, 12k Flex; Bio-Rad：CFX96, CFX384, iCycler iQ, iQ5, MyiQ, MiniOpticon, Opticon, Opticon 2, Chromo4; Eppendorf：Mastercycler ep realplex, realplex 2 s; Qiagen：Corbett Rotor-Gene Q, Rotor-Gene 3000, Rotor-Gene 6000; Roche Applied Science：LightCycler 480, LightCycler 2.0; Lightcycle r 96; Thermo Scientific：PikoReal Cycler; Cepheid：SmartCycler; Illumina：Eco qPCR. |
Components
| Components No. | Name | 11205ES03 | 11205ES08 | 11205ES25 |
| 11205-A | Hieff Unicon™ qPCR TaqMan Probe Master Mix | 1 mL | 5×1 mL | 25 mL |
| 11205-B | 50×Low Rox | 40 μL | 200 μL | 1 mL |
| 11205-C | 50×High Rox | 40 μL | 200 μL | 1 mL |
Storage
The product should be stored at -25°C~-15℃ away from light for 18 months. Please avoid repeated freeze-thaw.
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