Description
The product is a premixed solution for 2× real-time quantitative PCR amplification by the probe method. The antibody hot-start UNICONTM DNA Polymerase used can effectively inhibit the amplification caused by the non-specific annealing of primers. At the same time, the formula optimizes the factors that can effectively inhibit non-specific PCR amplification and improve the amplification efficiency of PCR reaction, which is suitable for the amplification of low-concentration templates, so that quantitative PCR can obtain a good standard curve in a wide quantitative area.
Features
- High specificity
- High sensitivity
Applications
- Gene Expression
Specifications
| Hot Start | Built-in hot start |
| Detection method | Primer-probe detection |
| PCR method | RT-qPCR |
| Polymerase | Taq DNA polymerase |
| Type of sample | RNA |
| Application equipment | Applied Biosystems: 5700, 7000, 7300, 7700, 7900HT Fast, StepOne™, StepOne™ Plus; 7500, 7500 Fast, ViiA™7, QuantStudio™ 3 and 5, QuantStudio™ 6, 7, 12k Flex; Bio-Rad：CFX96, CFX384, iCycler iQ, iQ5, MyiQ, MiniOpticon, Opticon, Opticon 2, Chromo4; Eppendorf：Mastercycler ep realplex, realplex 2 s; Qiagen：Corbett Rotor-Gene Q, Rotor-Gene 3000, Rotor-Gene 6000; Roche Applied Science：LightCycler 480, LightCycler 2.0; Lightcycle r 96; Thermo Scientific：PikoReal Cycler; Cepheid：SmartCycler; Illumina：Eco qPCR. |
Components
| Components No. | Name | 11205ES03 | 11205ES08 | 11205ES25 |
| 11205-A | Hieff Unicon™ qPCR TaqMan Probe Master Mix | 1 mL | 5×1 mL | 25 mL |
| 11205-B | 50×Low Rox | 40 μL | 200 μL | 1 mL |
| 11205-C | 50×High Rox | 40 μL | 200 μL | 1 mL |
Storage
The product should be stored at -25°C~-15℃ away from light for 18 months. Please avoid repeated freeze-thaw.
[1] Huang T, Li X, Wang F, et al. The CREB/KMT5A complex regulates PTP1B to modulate high glucose-induced endothelial inflammatory factor levels in diabetic nephropathy. Cell Death Dis. 2021;12(4):333. Published 2021 Mar 29. doi:10.1038/s41419-021-03629-4(IF:8.469)
[2] Ma H, Lin J, Li L, et al. Formaldehyde reinforces pro-inflammatory responses of macrophages through induction of glycolysis. Chemosphere. 2021;282:131149. doi:10.1016/j.chemosphere.2021.131149(IF:7.086)
[3] Lu L, Li X, Zhong Z, et al. KMT5A downregulation participated in High Glucose-mediated EndMT via Upregulation of ENO1 Expression in Diabetic Nephropathy. Int J Biol Sci. 2021;17(15):4093-4107. Published 2021 Oct 3. doi:10.7150/ijbs.62867(IF:6.582)
[4] Lu L, Zhong Z, Gu J, Nan K, Zhu M, Miao C. ets1 associates with KMT5A to participate in high glucose-mediated EndMT via upregulation of PFN2 expression in diabetic nephropathy. Mol Med. 2021;27(1):74. Published 2021 Jul 8. doi:10.1186/s10020-021-00339-7(IF:6.354)
[5] Li X, Lu L, Hou W, et al. The SETD8/ELK1/bach1 complex regulates hyperglycaemia-mediated EndMT in diabetic nephropathy. J Transl Med. 2022;20(1):147. Published 2022 Mar 29. doi:10.1186/s12967-022-03352-4(IF:5.531)
[6] Shen X, Chen X, Wang J, et al. SET8 suppression mediates high glucose-induced vascular endothelial inflammation via the upregulation of PTEN. Exp Mol Med. 2020;52(10):1715-1729. doi:10.1038/s12276-020-00509-3(IF:5.418)
[7] Qi J, Chen X, Wu Q, et al. Fasting Induces Hepatocellular Carcinoma Cell Apoptosis by Inhibiting SET8 Expression. Oxid Med Cell Longev. 2020;2020:3985089. Published 2020 Mar 19. doi:10.1155/2020/3985089(IF:5.076)
[8] Zeng W, Ren J, Li Z, et al. Levistolide A Inhibits PEDV Replication via Inducing ROS Generation. Viruses. 2022;14(2):258. Published 2022 Jan 27. doi:10.3390/v14020258(IF:5.048)
[9] Li D, Ma H, Shu Q, et al. Arsenite inhibits M2a polarization of macrophages through downregulation of peroxisome proliferator-activated receptor gamma [published online ahead of print, 2022 Jun 28]. Toxicol Appl Pharmacol. 2022;450:116142. doi:10.1016/j.taap.2022.116142(IF:4.460)
[10] Long J, Liu L, Zhou X, Lu X, Qin L. HLA-DQB1-AS1 Promotes Cell Proliferation, Inhibits Apoptosis, and Binds with ZRANB2 Protein in Hepatocellular Carcinoma. J Oncol. 2022;2022:7130634. Published 2022 May 11. doi:10.1155/2022/7130634(IF:4.375)
[11] Hou W, Lu L, Li X, Sun M, Zhu M, Miao C. c-Myc participates in high glucose-mediated endothelial inflammation via upregulation of IRAK1 expression in diabetic nephropathy. Cell Signal. 2022;92:110263. doi:10.1016/j.cellsig.2022.110263(IF:4.315)
[12] Ma H, Ding Z, Xie Y, et al. Methylglyoxal produced by tumor cells through formaldehyde-enhanced Warburg effect potentiated polarization of tumor-associated macrophages. Toxicol Appl Pharmacol. 2022;438:115910. doi:10.1016/j.taap.2022.115910(IF:4.219)
[13] Cao N, Tang Z, Zhang X, et al. Development and Application of a Triplex TaqMan Quantitative Real-Time PCR Assay for Simultaneous Detection of Feline Calicivirus, Feline Parvovirus, and Feline Herpesvirus 1. Front Vet Sci. 2022;8:792322. Published 2022 Feb 8. doi:10.3389/fvets.2021.792322(IF:3.412)
[14] Qi J, Wu Q, Cheng Q, Chen X, Zhu M, Miao C. High Glucose Induces Endothelial COX2 and iNOS Expression via Inhibition of Monomethyltransferase SETD8 Expression. J Diabetes Res. 2020;2020:2308520. Published 2020 Feb 23. doi:10.1155/2020/2308520(IF:2.965)
Payment & Security
Your payment information is processed securely. We do not store credit card details nor have access to your credit card information.
Inquiry
You may also like
FAQ
The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
Certain applications may require additional third-party intellectual property rights.
Yeasen is dedicated to ethical science, believing our research should address critical questions while ensuring safety and ethical standards.