Hifair™ V Reverse Transcriptase is an updated version of Hieff™M-MLV (H-) Reverse Transcriptase obtained by genetic engineering technology. It has higher cDNA synthesis ability, thermal stability, and reaction temperature limit (up to 60°C) than Hieff™ M-MLV (H-) Reverse Transcriptase. The synthesized cDNA product is up to 10kb. Hifair™ V Reverse Transcriptase enhances the affinity of the templates and is suitable for reverse transcription of RNA templates with complex secondary structures or low-copy genes.

Features 

Outstanding thermal resistance.

Suitable for RT-PCR/RT-qPCR applications.

Glycerol-free, more suitable for lyophilization.

Performance demonstration

1. Capable of withstanding a reaction temperature of 58°C, it is suitable for the reverse transcription of complex RNA templates.

Figure 1. The Reverse Transcriptase of Hifair™  V Reverse Transcriptase and the brand T* were incubated at 55℃ and 58℃ respectively for 30min to detect the residual enzyme activity. The results showed that Hifair™ V Reverse Transcriptase could still maintain 70% activity after incubation at 58℃ for 30min, which was much higher than that of the brand T*.

2. The detection rate of RT-qPCR was better than that of other brands, and the detection range was 1 pg-1 μg.

Figure 2. Using 1 pg-1 μg (7 gradients) total RNA of 293T cells as template, reverse transcription was performed using Hifair™  V one-step RT-gDNA digestion SuperMix for qPCR(Cat#11142), V*, T*. The obtained cDNA was quantitatively analyzed by qPCR. The results showed that the sensitivity of 11142ES could reach 1 pg, which was better than that of other brands.