Description
CRISPR/Cas9 is a technique in which RNA-directed Cas nuclease to make specific DNA modifications to targeted genes, which is an acquired immune defense mechanism evolved by bacteria and archaebacteria in response to attacks by phages and foreign plasmids. In this system, the Cas9 nuclease is guided with artificially optimized single guide RNA (sgRNA) to cut double-stranded DNA (dsDNA) at the target site causing DNA breaks. DNA is repaired with the non-homologous end joining or Homology directed repair mechanism in vivo, resulting in frameshift mutation, replacement or deletion of genes.
HifairTM Precision sgRNA Synthesis Kit uses a T7 RNA polymerase mixture to efficiently transcribe spCas9 sgRNA. The kit contains a specific sequence that is recognized by the Cas9 protein and acts as a scaffold. A portion of the sequence can be partially overlapped with the target specific sequence designed by the user. The complementary strands are filled by DNA polymerase to generate dsDNA templates for transcription. Using its supplied reagent and user-designed specific DNA sequences, the kit can obtain 20-100 μg of functional sgRNA within 4 hours in a single tube reaction.
Features
High synthesis yield: In a single-tube reaction over 4 hours, the yield of sgRNA can reach 20 - 100 μg.
Wide application range: High yields can be obtained for different types of sgRNA.
High purity: The synthesized sgRNA has a single band.
High activity: The synthesized sgRNA can effectively guide Cas9 to cleave the target DNA.
Applications
CRISPR/Cas gene editing
Components
|
Components No. |
Name |
11355ES05 |
11355ES25 |
11355ES50 |
11355ES60 |
|
11355-A |
10×sgRNA Enzyme Mix |
10 μL |
50 μL |
100 μL |
200 μL |
|
11355-B |
10×sgRNA Reaction Buffer |
10 μL |
50 μL |
100 μL |
200 μL |
|
11355-C |
2×Canace Enzyme Mix |
62.5 μL |
312.5 μL |
625 μL |
1.25 mL |
|
11355-D |
Scaffold Template |
6.25 μL |
31.25 μL |
62.5 μL |
125 μL |
|
11355-E |
NTPs (25 mM each) |
40 μL |
200 μL |
400 μL |
800 μL |
|
11355-F |
Control sgRNA Oligo (10 μM) |
2 μL |
10 μL |
20 μL |
40 μL |
Shipping and Storage
This product should be stored at -25 ~ -15oC for 2 years.
Figures
Figure 1.The synthesis amount of sgRNA at different time points
Figure 2.The synthesis yield of sgRNA with different sequences
Figure 3.The purity of synthesized sgRNA (determined by Agilent 2100)
Figure 4.The cleavage efficiency chart of sgRNA
Documents:
Safety Data Sheet
Manuals
11355_Manual_Ver.EN20240710.pdf
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FAQ
The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
Certain applications may require additional third-party intellectual property rights.
Yeasen is dedicated to ethical science, believing our research should address critical questions while ensuring safety and ethical standards.