Description
Firefly luciferase is a protein with a molecular weight of approximately 61 kDa. In the presence of ATP, magnesium ions, and oxygen, it can catalyze the oxidation of luciferin to oxyluciferin, during which a bioluminescence with a wavelength of around 560 nm is emitted.
Detection principle as shown in the figure:
Figure 1: Schematic diagram of the firefly luciferase detection principle
YEASEN Biotech's Bio-One Step Luciferase Reporter Gene Detection Kit is characterized by its ease of operation and high signal strength. There is no need to wash or collect cells before the experiment; simply add the reagent directly to the culture medium, and the lysis and detection of cells are completed in one step, saving experimental time. This kit achieves a relative balance between signal strength and stability. It maintains a relatively high signal strength while keeping the signal intensity relatively stable over a certain period, ensuring that the experimental results are accurate and reliable. The half-life typically reaches over 1 hour. The product has a wide linear range for sample determination. In a 96-well plate, it can exhibit a good linear relationship within a range of 200,000 cells.
Features
Long signal half-life: The signal half-life is 1 hour, meeting the needs of high-throughput experiments.
Strong fluorescence signal: The signal value can reach 8 orders of magnitude, with a large detection window.
Convenient operation: One-step procedure, avoiding complicated experimental processes.
Applications
Signaling Pathway Research
Candidate Drug Screening
T Cell Killing Assay
Specifications
Form |
liquid |
Luciferase |
Firefly |
Signal Half-life |
1 h |
Number of Product Components |
1 |
Workflow |
One Step |
Components
Components No. |
Name |
11411ES60 |
11411ES80 |
11411ES81 |
11411 |
Bio-One Step Luciferase Assay Kit |
10 mL |
10×10 mL |
100 mL |
Shipping and Storage
Bio-One Step Luciferase Assay Kit products should be stored at -80℃ for 1 years.
Figures
Figure1. YEASEN Bio-One Step has better stability and higher signal values compared to its competitors.
Documents:
Safety Data Sheet
Manuals
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FAQ
The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
Certain applications may require additional third-party intellectual property rights.
Yeasen is dedicated to ethical science, believing our research should address critical questions while ensuring safety and ethical standards.