Description
Firefly luciferase is a protein with a molecular weight of approximately 61 kDa. In the presence of ATP, magnesium ions, and oxygen, it can catalyze the oxidation of luciferin to oxyluciferin, during which a bioluminescence with a wavelength of around 560 nm is emitted.
Detection principle as shown in the figure:
Figure 1: Schematic diagram of the firefly luciferase detection principle
YEASEN Biotech's Bright-One Step Luciferase Assay Kit features easy operation, high sensitivity, and strong signal intensity. It is suitable for experiments that require high sensitivity. There is no need to wash or collect cells before the experiment, and it can be used directly. The lysis and detection of cells are completed in one step, eliminating the need to mix reagents, saving experimental time.
Features
Long signal half-life: The signal half-life is 30 min, meeting the needs of high-throughput experiments.
Strong fluorescence signal: The signal value can reach 8 orders of magnitude, with a large detection window.
Convenient operation: One-step procedure, avoiding complicated experimental processes.
Applications
Signaling Pathway Research
Candidate Drug Screening
T Cell Killing Assay
Specifications
|
Form |
liquid |
|
Luciferase |
Firefly |
|
Signal Half-life |
30 min |
|
Number of Product Components |
1 |
|
Workflow |
One Step |
Components
|
Components No. |
Name |
11412ES60 |
11412ES80 |
11412ES81 |
|
11412 |
Bright -One Step Luciferase Assay Kit |
10 mL |
10×10 mL |
100 mL |
Shipping and Storage
Bright -One Step Luciferase Assay Kit products should be stored at -80℃ for 1 years.
Figures
Figure1. YEASEN Bright -One Step has better stability and higher signal values compared to its competitors.
Documents:
Safety Data Sheet
Manuals
Payment & Security
Your payment information is processed securely. We do not store credit card details nor have access to your credit card information.
Inquiry
You may also like
FAQ
The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
Certain applications may require additional third-party intellectual property rights.
Yeasen is dedicated to ethical science, believing our research should address critical questions while ensuring safety and ethical standards.