Description
Firefly luciferase is a protein with a molecular weight of approximately 61 kDa. In the presence of ATP, magnesium ions, and oxygen, it can catalyze the oxidation of luciferin to oxyluciferin, during which a bioluminescence with a wavelength of around 560 nm is emitted.
Detection principle as shown in the figure:
Figure 1: Schematic diagram of the firefly luciferase detection principle
YEASEN Biotech's Steady-One Step Luciferase Reporter Gene Detection Kit is characterized by its ease of operation and a long half-life of over 5 hours, with minimal luminescence value changes in the first 2 hours. It is particularly suitable for ultra-high throughput or 384-well plate detection. There is no need to wash or collect cells before the experiment; the reagent can be used directly, with cell lysis and detection completed in one step, eliminating the need for mixing reagents, thus saving experimental time. This kit achieves a relative balance between signal strength and stability, maintaining a relatively high signal strength while keeping the signal intensity relatively stable over a certain period, ensuring that the experimental results are accurate and reliable. The half-life typically reaches over 1 hour. The product has a wide linear range for sample determination. In a 96-well plate, it can exhibit a good linear relationship within a range of 200,000 cells.
Features
Long signal half-life: The signal half-life is 5 hour, meeting the needs of high-throughput experiments.
Strong fluorescence signal: The signal value can reach 7 orders of magnitude, with a large detection window.
Convenient operation: One-step procedure, avoiding complicated experimental processes.
Applications
Signaling Pathway Research
Candidate Drug Screening
T Cell Killing Assay
Specifications
|
Form |
liquid |
|
Luciferase |
Firefly |
|
Signal Half-life |
5 h |
|
Number of Product Components |
1 |
|
Workflow |
One Step |
Components
|
Components No. |
Name |
11413ES60 |
11413ES80 |
11413ES81 |
|
11413 |
Steady -One Step Luciferase Assay Kit |
10 mL |
10×10 mL |
100 mL |
Shipping and Storage
Steady -One Step Luciferase Assay Kit products should be stored at -80℃ for 1 years.
Figures
Figure1. YEASEN Steady -One Step has better stability and higher signal values compared to its competitors..
Documents:
Safety Data Sheet
Manuals
Payment & Security
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