Description
Hieff NGSTM EvoMax RNA Library Prep Kit (dNTP) is a premixed, actinomycin D free and strand-specific total RNA sequencing library prep kit compatible with Illumina and MGI platforms. This product is available in two types: tube or sealing kit, and this kit is more convenient whether by Automated Liquid Handling Device or manual manipulation . The kit contains RNA fragmentation reagents, reverse transcription reagents, strand-specific ds-cDNA synthesis reagents, and library amplification reagents. It can be linked with the mRNA purification kit or the rRNA removal kit to do mRNA or lncRNA research. This product optimizes the reverse transcription module to obtain a high chain specificity library without actinomycin D, which ensures the safety of the experimenters to a greater extent. All reagents were subjected to strict quality control and functional validation to maximize the stability and repeatability of the library preparation.
Features
- Simplied components of EvoMax RNA Library Prep Kit
- The layout of plate reagent group,Improve automatability with fewer handling steps
- Increase sensitivity with FFPE and low-input samples
- Generate high-quality libraries with rapid, easily automated workflows
Applications
- Gene Expression Analysis
- Alternative Splicing Studies
- Non-Coding RNA Discovery
- Transcriptional Regulation
Specifications
|
Cat.No. |
12341ES24/12341ES96/12341ES97/12341ES98 |
|
Size |
24 T / 96 T / 96 T (automation) / 96 T (plate) |
Components
|
Components No. |
Name |
12341ES24 |
12341ES96 |
12341ES97 (automation ) |
12341ES98 (Plate )** |
|
12341-A |
Frag/Prime Buffer |
450 μL |
2×900 μL |
2×1064 μL |
266×8 μL |
|
12341-B |
1st Reaction Module 2.0 |
192 μL |
768 μL |
960 μL |
120×8 μL |
|
12341-C |
2nd Reaction Module(dNTP) |
840 μL |
3×1120 μL |
3×1280 μL |
480×8 μL |
|
12341-D |
Ligation Reaction Module |
840 μL |
3×1120 μL |
3×1280 μL |
480×8 μL |
|
12341-E |
2×Super Canace® II High-Fidelity Mix |
600 μL |
2×1200 μL |
2×1360 μL |
340×8 μL |
|
* |
Primer Mix* |
/ |
/ |
/ |
/ |
Note: * The notation indicates that the component is not included in the kit. Primer mix is required if the complete adaptors are used, otherwise it isn’t. This kit is compatible with Illumina and MGI platforms, but need additional primer mix (Cat # 13334 Primer Mix for MGITM and Cat # 13335 Primer Mix for Illumina®) for Illumina® or MGI platform if the complete adaptors are used.
** See the following diagram for the layout of plate reagent group.
Figure: reagent layout of sealing-plate library kit
Shipping and Storage
This product should be stored at -25~-15℃ for 1 year.
Figures
|
Types of RNA detected |
Protein-coding (exonic mRNA) |
Protein-coding (exonic; mRNA) |
Protein-coding (exonic; mRNA) |
Protein-coding (exonic; mRNA) |
|
RNA enrichment method |
Bead-based poly(A) capture |
Ribosomal and globin depletion |
Ribosomal and globin depletion |
none |
|
RNA input |
10 ng-4 μg |
100 ng-1 μg |
100 ng-1 μg |
10 ng-1 μg |
|
Library Prep Time |
60 min +202 min |
135 min + 202 min |
135 min+202 min |
202 min |
|
Species compatibility |
Any eukaryote |
human, mouse and rat |
plant |
Any |
|
Includes reagents for: |
mRNA Capture (Cat#12629ES) EvoMax RNA Library Prep
|
rRNA depletion(Cat#12257) EvoMax RNA Library Prep |
rRNA depletion(Cat#12254) EvoMax RNA Library Prep |
EvoMax RNA Library Prep |
Documents:
Safety Data Sheet
Manuals
12341_Manual_Ver. EN20250205_EN.pdf
Citations & References:
Payment & Security
Your payment information is processed securely. We do not store credit card details nor have access to your credit card information.
Inquiry
You may also like
FAQ
The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
Certain applications may require additional third-party intellectual property rights.
Yeasen is dedicated to ethical science, believing our research should address critical questions while ensuring safety and ethical standards.