Description
Hieff NGSTM FFPE DNA Repair Reagent is specifically designed for FFPE samples to repair varying degrees of damage in low-quality FFPE DNA, enhancing library yield and sequencing quality. This product can be directly used with Yeasen Hieff NGSTM Library Preparation Kits (Cat#12197/Cat# 12927) and the A-tailing module (Cat# 12608), with compatible systems that can repair 5 ng to 1 μg of fragmented FFPE DNA. Additionally, this product is also suitable for use with Yeasen Fragmentation Enzyme-based Library Preparation Kit.
Features
High Repair Efficiency: Provides higher repair efficiency for FFPE DNA.
Streamlined Workflow: Simple operation with strong adaptability.
High Compatibility: Enzyme components can be used with the end-repair and A-tailing system of library preparation kits, requiring only a single buffer to complete FFPE DNA repair and end-repair/dA-tailing.
Applications
Repair of damaged low-quality FFPE samples
Library preparation for low-quality FFPE samples
Specifications
|
Cat.No. |
12606ES24/ 12606ES96 |
|
Size |
24 T /96 T |
Components
|
Components No. |
Name |
12606ES24 |
12606ES96 |
|
12606-A |
FFPE DNA Repair Mix |
96 μL |
384 μL |
|
12606-B |
Endprep Buffer 2.0 |
144 μL |
576 μL |
Shipping and Storage
This product should be stored at -25~-15℃ for 1 year.
Figures
Figure1. The impact of FFPE repair reagents on library yield
During the library construction process, two sets of experiments were conducted to compare the same samples. One group included a repair reagent in the end-repair and A-tailing step, while the other group did not. The experimental results showed that the FFPE repair reagent significantly increased library yield for low-quality FFPE samples by repairing the samples, whereas its effect on high-quality FFPE samples was less pronounced.
Documents:
Safety Data Sheet
Manuals
12606_Manual_Ver.EN20250206.pdf
Citations & References:
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FAQ
The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
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