Description
dsDNA HS Assay Kit is a fast, sensitive and accurate double-stranded DNA (dsDNA) fluorescence quantitative detection kit. The kits include concentrated assay reagents, dilution buffer, and DNA standards. This kit is highly selective for dsDNA over RNA and is accurate for initial sample concentration from 10 pg/μL to 100 ng/μL. It is an ideal kit for DNA sample quantification such as NGS input DNA quantification and DNA library quantification. The kit has good tolerance to conventional contaminants such as salts, free nucleotides, solvents, detergents, and proteins.
Features
- High sensitive: the concentrations ranging from 10 pg/µ l to 100 ng/µL of dsDNA can be accurately quantified;
- High specificity: this product is highly selective to dsDNA and is not affected by RNA, has good tolerance to some conventional pollutants, such as salt, free nucleotide, protein, solvent, detergent, etc;
- Easy to use: the dsDNA sample is to be tested and test it with a Qubit fluorometer or Fluorescent microplate reader at room temperature
Applications
- dsDNA quantification with Qubit or Fluorescent microplate reader
- NGS library quantification
Specifications
| Assay | dsDNA Quantitation |
| Excitation/Emission | 480/520 |
| For Use With (Equipment) | Qubit Fluorometer, Microplate Reader |
| No. of Reactions | 100T/500T |
| Product Line | Qubit Quantitation |
| Quantitation Range | 10 pg/μL to 100 ng/μL |
| Sample Volume | 1 μL to 20 μL |
| Detection Method | Fluorescent |
Components
| Components No. | Name | Concentration | 12640ES60 (100T) | 12640ES76 (500T) |
| 12640-A | dsDNA Reagent | 200×concentrate in DMSO | 250 μL | 1.25 mL |
| 12640-B | dsDNA Buffer | Not applicable | 50 mL | 250 mL |
| 12640-C | dsDNA Standard 1 | 0 ng/μL in TE buffer | 1 mL | 5×1 mL |
| 12640-D | dsDNA Standard 2 | 10 ng/μL in TE buffer | 1 mL | 5×1 mL |
Shipping and Storage
All the components are shipped with an ice pack and can be stored at 2-8℃ away from light for one year.
Figure
Product stability test. Super stability, high relevance of test results within the validity period
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