Description
ssDNA Assay Kit is a simple, sensitive, and accurate single-strand DNA (ssDNA) fluorescence quantitative detection kit with a good linear relationship between 1-200 ng. This kit contains a fluorescence detection reagent, buffer solution, and related ssDNA standards. Before use, dilute the buffer solution of fluorescence detection reagent into a working solution, and then add the ssDNA sample to be tested, then use the fluorescence microplate or Qubit Read with a fluorometer. The selectivity of this kit to single-stranded DNA is not higher than that to double-stranded DNA, but it has good tolerance to conventional pollutants such as proteins, salts, detergents, etc.
Features
- TFast and accurate ssDNA quantification
- High sensitivity, there is a good linear relationship in the low-concentration range
- Very suitable for the MGI platform library
Applications
- ssDNA quantification
- MGI Circular library and DNB quantification
- BS product quantification
Specifications
| Assay | ssDNA Quantitation |
| Excitation/Emission | 500/525 |
| For Use With (Equipment) | Qubit Fluorometer，fluorescence microplate |
| No. of Reactions | 100T/500T |
| Product Line | Qubit Quantitation |
| Quantitation Range | 1-200 ng, 50pg/μL - 200ng/μL |
| Sample Volume | 1 μL to 20 μL |
| Detection Method | Fluorescent |
Components
| Name | Concentration | 12646ES60 (100T) | 12646ES60 (500T) |
| ssDNA Reagent | 200× in DMSO | 250 μL | 1250 μL |
| ssDNA Buffer | Not applicable | 50 mL | 250 mL |
| ssDNA Standard 1 | 0 ng/μL in TE buffer | 1 mL | 5×1 mL |
| ssDNA Standard 2 | 20 ng/μL in TE buffer | 1 mL | 5×1 mL |
Shipping and Storage
All the components are shipped with ice packs and can be stored at 2℃~8℃ away from light for 6 months. Please avoid repeated freeze-thawing.
Figures
Figure 1 Linearity Performance
Test Sample: ssDNA standards; Conclusion: Good linearity within 0-200ng
Figure 2 Comparison of measured values of different batches and original Thermophile products
The stability of Yeasen（Cat # 12645）three batches of products is good, which can accurately quantify different types of ssDNA samples, and consistent with the competitive products(Thermo Q10212)
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The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
Certain applications may require additional third-party intellectual property rights.
Yeasen is dedicated to ethical science, believing our research should address critical questions while ensuring safety and ethical standards.