Description
T4 DNA Polymerase catalyzes the synthesis of DNA in the 5'→3' direction and requires the presence of a template and a primer. This enzyme has a 3'→5' exonuclease activity, but it does not have a 5'→3' exonuclease function.
Applications
3´ overhang removal to form blunt ends
5´ overhang fill-in to form blunt ends
Second strand synthesis in site-directed mutagenesis
Probe labeling using replacement synthesis
Cloning of PCR products without reliance on ligation reactions
Specifications
|
Source |
Bacteriophage T4 |
|
Molecular Weight |
103 kDa |
|
Concentration |
5 U/μL |
|
Unit Definition |
One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of deoxyribonucleotide triphosphates (dNTPs) into a polynucleotide within 30 minutes at 37°C |
Components
|
Name |
12901ES60 |
12901ES76 |
12901ES90 |
12901ES08 |
|
|
12901-A |
T4 DNA Polymerase(5 U/μL) |
20 μL |
100 μL |
400 μL |
1 mL |
|
12901-B |
10 × Blue Buffer |
100 μL |
250 μL |
500 μL |
1.25 mL |
Shipping and Storage
This product should be stored at -25 ~ -15℃ for 1 year.
Documents:
Safety Data Sheet
Manuals
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FAQ
The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
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