Description
T4 DNA Polymerase catalyzes the synthesis of DNA in the 5'-3' direction and requires the presence of templates and primers, and T4 DNA Polymerase has 3'→5' exonuclease activity but does not have 5'→3' exonuclease activity.
Product Components
|
Component number |
Components |
Cat#/Size |
||
|
12901ES60 |
12901ES76 |
12901ES90 |
||
|
12901-A |
T4 DNA polymerase (5 U/μL) |
20 μL |
100 μL |
400 μl |
|
12901-B |
10 × Blue Buffer |
100 μl |
250 μl |
500 μl |
Shipping and Storage
The product is shipped with dry ice and can be stored at -20°C for one year.
Cautions
1. For your safety and health, please wear lab coats and disposable gloves for operation.
2. For research use only!
Product Application
T4 DNA Polymerase can be used as followed:
1. Catalyze the blunt ends of the 5' or 3' protruding ends of DNA.
2. Labeled DNA probe synthesis by a displacement reaction.
3. Synthesis of the second chain during fixed-point mutation.
4. Cloning of PCR products that do not depend on ligation reactions.
Unit Definitions
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into polynucleotides in 30 minutes at 37°C.