Description
T4 Polynucleotide Kinase (T4 PNK) is a 5'-hydroxyl kinase that facilitates the transfer of the γ-phosphate from ATP to the 5'-hydroxyl termini of oligo- or polynucleotide chains, including double-stranded and single-stranded DNA or RNA, as well as to 3'-monophosphate nucleotides. This transfer is reversible. T4 PNK also possesses 3'-phosphatase activity, which dephosphorylates 3'-phosphorylated oligonucleotide termini, deoxy 3'-monophosphate nucleotides, and deoxy 3'-diphosphate nucleotides. In the presence of ADP, T4 PNK can act as a 5'-phosphatase, promoting the exchange of 5'-phosphate groups on 5'-phosphorylated oligo-or polynucleotides with ATP. This enzyme is widely used for the construction of DNA libraries and for end labeling to generate probes.
Applications
Phosphorylation of the 5' ends of DNA or RNA for subsequent ligation
Terminal labeling of DNA or RNA to serve as probes and for sequencing purposes
5' phosphorylation of mononucleotides with a phosphorylated 3' end to prepare pNp substrates for attachment to the 3' ends of DNA or RNA
Labeling the 5' ends of oligonucleotides that possess a 3' phosphate group
Specifications
|
Source |
Bacteriophage T4 |
|
Molecular weight |
36.78 kDa |
|
Concentration |
10 U/μL |
|
Unit definition |
One unit is defined as the the amount required to catalyze the incorporation of 1 nmol of [γ-32P] ATP into a reaction within 30 minutes at 37°C |
Components
|
Components No. |
Name |
12902ES76 |
12902ES86 |
12902ES92 |
|
12902-A |
T4 polynucleotide Kinase(10 U/μL) |
50 μL |
250 μL |
1 mL |
|
12902-B |
10×T4 PNK Buffer |
500 μL |
1 mL |
2×1 mL |
[Note]: The 10×T4 PNK Buffer does not contain ATP; customers need to add it themselves, with a reference final concentration of 1 mM, or use the T4 DNA ligase buffer.
Shipping and Storage
This product should be stored at -25 ~ -15℃ for 1 year.
Documents:
Safety Data Sheet
Manuals
Payment & Security
Your payment information is processed securely. We do not store credit card details nor have access to your credit card information.