Description
T4 Polynucleotide Kinase (T4 PNK) is a polynucleotide 5'-hydroxyl kinase that catalyzes the transfer of the γ-phosphate from ATP to the 5'-OH group of oligonucleotide chains (double- or single-stranded DNAs or RNAs) and nucleoside 3'-monophosphates, and the reaction is reversible. Has 3'- phosphatase activity, hydrolyzes the 3'-phosphate group from the 3'-phosphate end of oligonucleotides, deoxy3'-monophosphate nucleosides and deoxy3'-diphosphate nucleosides. In the presence of ADP, T4 PNK has 5'- phosphatase activity, catalyzing the exchange of 5'-P-oligo/polynucleotide and ATP terminal 5'-phosphate groups. It is suitable for DNA library construction and probes end labeling.
Product Components
|
Component |
Size |
|||
|
12902ES76 |
12902ES86 |
12902ES92 |
||
|
12902-A |
T4 Polynucleotide Kinase（10 U/μL） |
50 μL |
250 μL |
1 mL |
|
12902-B |
10× T4 PNK Buffer |
500 μL |
1 mL |
2 × 1 mL |
[Note]: 10× T4 PNK Buffer does not contain ATP, customers need to add it by themselves, refer to the final concentration of 1 mM, or use T4 DNA ligase buffer.
Shipping and Storage
All the components are shipped with dry ice and can be stored at -20°C for one year.
Cautions
1. For your safety and health, please wear lab coat and disposable gloves for operation.
2. This product is for research use ONLY！
Applications
Phosphorylation of DNA or RNA 5´-end for ligation;
End-labeling of DNA or RNA for use as probes and DNA sequencing;
5'-phosphorylation of oligonucleotides that has been phosphorylated at the 3´-end to prepare a pNp substrate for addition to the 3´ end of DNA or RNA;
Labeling 5'-termini of oligonucleotides with a 3'-phosphate group.
Unit Definition
The required amount of T4 Polynucleotide Kinase to catalyze the recombination reaction of 1 nmol [γ-32P]-ATP in 30 min at 37 °C.