Description
DNA Polymerase I, in the presence of template and primer (DNA or RNA), uses dNTP as a substrate to synthesize DNA complementary to the template in the 5'→3' direction. The molecular weight of this enzyme is about 109,000 Da, and it has double-strand-specific 5'→3' exonuclease activity and single-strand-specific 3'→5' exonuclease activity.
Applications
With DNase I (Cat#10607) for nick translation reaction
With RNase H (Cat#12906) to synthesize the second strand of cDNA
Specifications
|
Concentration |
10 U/μL |
|
Unit Definition |
One unit is defined as the amount of enzyme required to degrade 1 µg of circular plasmid within a 10-minute period at 37°C in a 100 µl reaction system |
Components
|
Components No. |
Name |
12903ES60 |
12903ES76 |
12903ES92 |
|
12903-A |
DNA polymerase I (10 U/μL) |
50 μL |
250 μL |
1 mL |
|
12903-B |
10×DNA polymerase I Reaction Buffer |
1 mL |
1×5 mL |
4×5 mL |
Shipping and Storage
This product should be stored at -25 ~ -15℃ for 2 years.
Documents:
Safety Data Sheet
Manuals
Payment & Security
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