Description
DNA Polymerase I, in the presence of template and primer (DNA or RNA), uses dNTP as a substrate to synthesize DNA complementary to the template in the 5'→3' direction. The molecular weight of this enzyme is about 109,000 Da, and it has double-strand-specific 5'→3' exonuclease activity and single-strand-specific 3'→5' exonuclease activity.
Applications
With DNase I (Cat#10607) for nick translation reaction
With RNase H (Cat#12906) to synthesize the second strand of cDNA
Specifications
|
Concentration |
10 U/μL |
|
Unit Definition |
One unit is defined as the amount of enzyme required to degrade 1 µg of circular plasmid within a 10-minute period at 37°C in a 100 µl reaction system |
Components
|
Components No. |
Name |
12903ES60 |
12903ES76 |
12903ES92 |
|
12903-A |
DNA polymerase I (10 U/μL) |
50 μL |
250 μL |
1 mL |
|
12903-B |
10×DNA polymerase I Reaction Buffer |
1 mL |
1×5 mL |
4×5 mL |
Shipping and Storage
This product should be stored at -25 ~ -15℃ for 2 years.
Documents:
Safety Data Sheet
Manuals
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The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
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