Description
DNA Polymerase I, in the presence of template and primer (DNA or RNA), uses dNTP as a substrate to synthesize DNA complementary to the template in the 5'—3' direction. The molecular weight of this enzyme is about 109,000 Da, and it has double-strand-specific 5'—3' exonuclease activity and single-strand-specific 3'—5 exonuclease activity. This enzyme can be used with DNase I (Cat#10607) for nick translation reaction and with RNase H (Cat#12906) to synthesize the second strand of cDNA.
Specifications
|
Cat.No. |
12903ES60/12903ES76/12903ES92 |
|
Size |
500 U/2,500 U/10,000U |
Components
|
Components No. |
Name |
12903ES60 |
12903ES76 |
12903ES92 |
|
12903-A |
DNA polymerase 1 |
50|iL |
250 |1L |
ImL |
|
12903-B |
10XDNAPolymerase 1 Reaction Buffer |
ImL |
1X5 mL |
4X5 mL |
Storage
This product should be stored at -25~-15°C for 2 years.
Notes
1.This product is for research use only.
2.Please operate with lab coats and disposable gloves, for your safety.