Description
RNase H (ribonuclease H) is an endoribonuclease that specifically hydrolyzes the phosphodiester bonds in RNA within DNA/RNA hybrids, but does not hydrolyze the phosphodiester bonds in single-stranded or double-stranded DNA or RNA.
Applications
Removal of mRNA before the second-strand synthesis of cDNA
Removal of RNA after the first-strand synthesis of cDNA in RT-PCR/RT-qPCR experiments
Removal of poly(A) after hybridization of Oligo(dT) with mRNA
Cleavage at specific sites in RNA
Specifications
|
Source |
Shigella flexneri |
|
Molecular Weight |
19.7 KDa |
|
Concentration |
5 U/μL |
|
Unit Definition |
One unit is defined as the the amount needed to hydrolyze RNA-DNA hybrids and produce 1 nmol of ribonucleotides in a 50 μL reaction system within 20 minutes at 37°C |
Components
|
Components No. |
Name |
12906ES60 |
12906ES76 |
12906ES90 |
|
12906-A |
RNase H(5 U/μL) |
20 μL |
100 μL |
1 mL |
|
12906-B |
10×RNase H Reaction Buffer* |
200 μL |
1 mL |
10×1 mL |
Note: *10×RNase H Reaction Buffer：750 mM KCl，500 mM Tris-HCl，30 mM MgCl2，100 mM Dithiothreitol，pH 8.3 @ 25℃。
Shipping and Storage
This product should be stored at -25 ~ -15℃ for 2 years.
Documents:
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