Description
RNase H (ribonuclease H) is an endoribonuclease that specifically hydrolyzes the phosphodiester bonds of RNA on hybrid DNA/RNA strands, but will not hydrolyze phosphates in single- and double-stranded DNA or RNA diester bonds.
Product Components
|
Components |
12906ES76 (500 U) |
12906ES90 (5000 U) |
|
|
12906-A |
RNase H |
100 μL |
1 mL |
|
12906-B |
10×RNase H Reaction Buffer* |
1 mL |
10×1 mL |
[Note]：*10×RNase H Reaction Buffer：750 mM KCl，500 mM Tris-HCl，30 mM MgCl2，100 mM Dithiothreitol，pH 8.3 @ 25℃.
Shipping and Storage
All the components are shipped with dry ice and can be stored at -20°C for two years.
Applications
1. Remove mRNA before cDNA double-strand synthesis;
2. In RT-PCR/RT-qPCR experiments, remove RNA after the synthesis of one strand of cDNA;
3. Remove poly(A) after Oligo(dT) hybridizes with mRNA;
4. Cleavage of RNA-specific sites.
Definition of enzymatic activity
In 50 μL reaction system, the amount of enzyme required to hydrolyze the RNA-DNA hybrid strand to generate 1 nmol of ribonucleotides was defined as 1 U at 37°C for 20 min.
Cautions
1) For your safety and health, please wear a lab coat and disposable gloves for operation.
2) This product is only for scientific research purposes!