Description
RNase H (ribonuclease H) is an endoribonuclease that specifically hydrolyzes the phosphodiester bonds in RNA within DNA/RNA hybrids, but does not hydrolyze the phosphodiester bonds in single-stranded or double-stranded DNA or RNA.
Applications
Removal of mRNA before the second-strand synthesis of cDNA
Removal of RNA after the first-strand synthesis of cDNA in RT-PCR/RT-qPCR experiments
Removal of poly(A) after hybridization of Oligo(dT) with mRNA
Cleavage at specific sites in RNA
Specifications
|
Source |
Shigella flexneri |
|
Molecular Weight |
19.7 KDa |
|
Concentration |
5 U/μL |
|
Unit Definition |
One unit is defined as the the amount needed to hydrolyze RNA-DNA hybrids and produce 1 nmol of ribonucleotides in a 50 μL reaction system within 20 minutes at 37°C |
Components
|
Components No. |
Name |
12906ES60 |
12906ES76 |
12906ES90 |
|
12906-A |
RNase H(5 U/μL) |
20 μL |
100 μL |
1 mL |
|
12906-B |
10×RNase H Reaction Buffer* |
200 μL |
1 mL |
10×1 mL |
Note: *10×RNase H Reaction Buffer：750 mM KCl，500 mM Tris-HCl，30 mM MgCl2，100 mM Dithiothreitol，pH 8.3 @ 25℃。
Shipping and Storage
This product should be stored at -25 ~ -15℃ for 2 years.
Documents:
Payment & Security
Your payment information is processed securely. We do not store credit card details nor have access to your credit card information.
Inquiry
You may also like
FAQ
The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
Certain applications may require additional third-party intellectual property rights.
Yeasen is dedicated to ethical science, believing our research should address critical questions while ensuring safety and ethical standards.