Description
The Hieff NGS TM ds-cDNA Synthesis Kit is a cDNA synthesis kit for RNA sequencing libraries on the IlluminaTM /MGITM sequencing platforms, containing efficient RNA reverse transcription reagents and conventional ds-cDNA synthesis reagents. It can be connected to the Hieff NGSTM OnePot Flash DNA Library Prep Kit (Yeasen Cat#12316ES) for subsequent library construction. All reagents provided in this kit have undergone strict quality control and functional validation, ensuring the stability and reproducibility of library construction to the greatest extent.
Features
Fast: Rapid cDNA Synthesis. No purification of the second-strand product. cDNA synthesis only takes 53 minutes.
Compatible: Compatible with 100 pg - 500 ng total RNA.
High-quality: Upgraded reverse transcription module, excellent pathogen detection, and strict background bacteria quality control.
Applications
- Double-stranded cDNA Synthesis
- Metatranscriptome Detection
- Pathogen RNA library Prep
Specifications
|
Cat.No. |
13488ES08 / 13488ES24 / 13488ES96 |
|
Size |
8 T / 24 T / 96 T |
Components
|
Components No. |
|
Name |
13488ES08 |
13488ES24 |
13488ES96 |
|
13488-A |
|
Random Primer |
8 μL |
24 μL |
212 μL |
|
13488-B |
|
1st Reaction Buffer |
64 μL |
192 μL |
1267 μL |
|
13488-C |
|
1st Strand Enzyme Mix |
16 μL |
48 μL |
320 μL |
|
13488-D |
|
2nd Reaction Buffer |
56 μL |
168 μL |
1034 μL |
|
13488-E |
|
2nd Strand Enzyme Mix |
24 μL |
72 μL |
473 μL |
Shipping and Storage
This product should be stored at -25~-15℃ for 1 year.
Figures
|
RNA input |
100 pg-100 ng |
RNA: 100 pg-100 ng |
|
ds-cDNA Synthesis |
ds-cDNA Synthesis (Cat#13488ES) |
ds-cDNA Synthesis with gDNA digester (Cat#13488ES) |
|
ds-cDNA Synthesis time |
53 min |
92 min |
|
Library Prep |
Flash DNA Library Prep (Cat#12316ES) |
Flash DNA Library Prep (Cat#12316ES) |
|
Library Prep Time |
75 min |
75 min |
|
Species compatibility |
RNA (prefer) or DNA/RNA mixture |
RNA or DNA/RNA mixture |
Documents:
Safety Data Sheet
Manuals
13488_Manual_Ver. EN20250205_EN.pdf
Citations & References:
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The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
Certain applications may require additional third-party intellectual property rights.
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