Description
Hieff NGS™ Complete Adapter Kit for Illumina is designed for use with DNA and RNA library preparation kits to prepare libraries for sequencing on Illumina Platforms. The complete Adapter Kit contains 2 sets. Each set has 48 adapters labelled with 8-nucleotide indexes (barcode) for multiplexed sequencing applications. Set 1 contains DNA Adapters 01-48. All the reagents provided in the kit have strictly quality controlled and functional verification, which ensures the stability and repetitiveness of the library preparation to the greatest extent.
Specifications
| Product Type | Adapter Kit |
| Libraries | DNA or RNA Library |
| Input DNA amount | 100 pg - 1000 ng |
| Input RNA amount | 10 ng-4 μg |
| Instrument compatibility | Illumina Platforms |
| Application | NGS library preparation |
| Quantity | 192/768 Reactions |
Components
| Components No. | Name | 13519ES04 (48×4T) | 13519ES16 (48×16T) |
| 13519 | DNA Adapter 001-048 | 14 μL each | 56 μL each |
Storage
-25℃ ~ -15℃ storage, valid for 18 months.
Figures
- The DNA library structure
Figure 1. The DNA library structure
- Sequence information
The sequences of DNA Adapter for Illumina:
Universal Adapter：
5’-AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT-3’
index Adapter：
5’-GATCGGAAGAGCACACGTCTGAACTCCAGTCACXXXXXXXX1ATCTCGTATGCCGTCTTCTGCTTG-3’
XXXXXXXX1 represent the 8-nucleotide indexes (barcodes), which used in Sample Sheet, are listed in the Table below:
Figure 2. Sequence information