Description
RT-LAMP Dye Assay Kit (UDG plus) contains fluorescent dye, Hieff™ Bst Plus DNA Polymerase, heat-resistant Hifair™ Ⅲ Reverse Transcriptase, MRI, and UDGas applied to LAMP reaction with RNA as template. This product is with high sensitivity and high specificity.
Features
- High Sensitivity:50 copies/T, 25 μL system;
- High stability: stable at 37°C for 5 days with good stability, and can withstand repeated freezing and thawing more than 10 times;
- Specificity: 48-well NTC has no peaks at all, and the specificity is strong;
Applications
- Rapid detection of pathogenic bacteria
- Rapid detection of pet pathogens
- On-site detection of animal husbandry
Specifications
Detection method | Fluorescent dye method |
PCR method | RT-LAMP |
Polymerase | Hieff™ Bst Plus DNA Polymerase |
Type of sample | RNA |
LOD | 50 copies/T |
Specificity | 100% |
Components
Components No. | Name | 13762ES60 (100 T) | 13762ES80 (1000 T) |
13762-A | Bst Buffer | 600 µL | 6 mL |
13762-B | Bst Enzyme Mix(UDG plus) | 100 µL | 1 mL |
Storage
Part 1: 13762-A should be protected from light and can be stored at -20°C for 6 months.
Part 2: 13762-B can be stored at -20°C for 6 months.
Figures
- High Sensitivity
Figure 1: Sensitivity Test
Red: 50 copies/T, response less than 15 minutes
Figure 1: The copy numbers of 50 copies/Tweres tested. The test results showed that within 15 minutes, the results of 50 copies/T performed better, and the detection rate reached 100%.
- Stability
Figure 2.Stability testing at the same temperature
Red: 37℃ 0 day Blue: 37℃ 1 day Green: 37℃ 3 days Purple: 37℃ 5 days
0 days at 37°C as a control and stability tests were carried out for 1, 3, and 5 days at the same temperature, indicating that it can still maintain a relatively stable effect at 37°C for 5 days.
Figure 3. -20℃ repeated freeze-thaw stability test
Red: Repeat freeze-thaw 0 times
Blue: Repeat freeze-thaw 5 times
Green: Repeat freeze-thaw 10 times
- Specificity
Figure 4. 48-well NTC-specific assay
48-well NTC detection was used in the specific detection of the product, and no peak was detected, indicating that the specificity was better.
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The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
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