Hieff UNICON™ HotStart E-Taq DNA Polymerase, Glycerol-free (5 U/μL) -14316ES

SKU: 14316ES80

Size: 1000 U
Price:
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Description

Hieff UNICONTM HotStart E-Taq DNA Polymerase, Glycerol-free is a hot start DNA polymerase with double blocking by double antibodies independently developed by the company. This product not only blocks the 5'→3' polymerase activity of Taq DNA polymerase, but also blocks the 5'→3' exonuclease activity. Heating for 30 seconds at the pre denaturation temperature can completely inactivate the antibody and release DNA polymerase activity and exonuclease activity. The double blocking characteristic can not only effectively prevent the nonspecific amplification caused by mismatch or primer dimer, but also effectively inhibit the decline of fluorescence signal caused by probe degradation, so as to make the in vitro detection reagent more stable during transportation or use at room temperature. This product contains no Glycerol and is specially designed for the preparation of lyophilized reagents.

Specifications

Cat.No.

14316ES76 / 14316ES80 / 14316ES92 / 14316ES97 / 14316ES98

Size

500 U / 1000 U / 10 KU / 50 KU / 100 KU

Components

Name

14316ES76

14316ES80

14316ES92

Hieff UNICONTM HotStart E-Taq DNA Polymerase, Glycerol-free (5 U/μL)

100 μL

200 μL

2×1 mL

Figure

Storage

This product should be stored at 2~8℃ for 1 years.


 

Instructions

1.Reaction Setup

Components

Volume (μL)

Final Concentration

2×Buffer

25

Primer/Probe mix

X

0.1 μmol/L-0.5 μmol/L

Hieff UNICONTM HotStart E-Taq DNA Polymerase, Glycerol-free (5 U/μL)

1.2

0.12 U/μL

DNA template

X

0.1-100 ng

ddH2O

up to 50

-

*According to the specific experimental application, the corresponding reaction Buffer should be prepared by oneself. The amount of DNA and primer concentration in the above table are recommended concentrations, and the optimal concentration can be adjusted according to the specific experimental situation.

  1. Thermal cycling protocol (2-Step cycling protocol)

Stage

Temperature

Time

Cycles

Pre-denaturation

95℃

5 min

1

Denaturation

95℃

15 sec

45

Annealing/Extension

60℃

30 sec

*The reaction temperature is adjusted according to the Tm value of the designed primers. Different qPCR instruments need different fluorescence signal acquisition time, please set according to the shortest time limit.

Notes

1.This product is for scientific research purposes only.

2.Please wear the necessary PPE, such lab coat and gloves, to ensure your health and safety.

Documents:

Manuals


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