The product is a hot start DNA polymerase with double blocking by double antibodies independently developed by the company. This product not only blocks the 5'→3' polymerase activity of Taq DNA polymerase, but also blocks the 5'→3' exonuclease activity. Heating for 30 seconds at the pre denaturation temperature can completely inactivate the antibody and release DNA polymerase activity and exonuclease activity. The double blocking characteristic can not only effectively prevent the nonspecific amplification caused by mismatch or primer dimer, but also effectively inhibit the decline of fluorescence signal caused by probe degradation, so as to make the in vitro detection reagent more stable during transportation or use at room temperature.

In addition, this product has been processed with the UCF.METM ultra-low residual process from Yeasen Biotechnology, resulting in extremely low residual levels of nucleases and host gDNA. With an optimized amplification buffer (e.g., Cat#16716ES), it can effectively reduce non-specific amplification caused by primer-probe hybridization during sample mixing, system warming, and long-term storage. This supports the development of a real time PCR system that allows for pre-mixing of primers and probes.

Features

• Low nuclease residue: No residual exonuclease, nicking enzyme, or RNase.
• Ultra-low Residual: E. coli DNA residue level < 0.02 copies/100 U, suitable for applications with more stringent background bacterial requirements.
• Super stability: All components of the qPCR system except the template were combined and incubated at 37 °C for 7 days without compromising performance.
• Batch-to-batch stability: The UCF.ME® ultra-clean molecular enzyme production platform, with strict quality control, ensures the uniformity, stability, and timely supply of the product.

Specifications

Components

Storage

This product should be stored at -25~-15℃ for 2 years.

Figures

01 Residue of E. coli gDNA ＜0.02 copies/100 U

E. coli gDNA residues of different batches of UCF.METM Taq enzyme (Cat#14321ES) were detected. The result showed that E. coli gDNA residues residues of Taq DNA polymerase  were well below 0.02 copies/100U.

Figure 1. Detection of E.coli gDNA residue of UCF.ME^TM Taq enzyme (Cat#14321ES)

02 Super stability：37℃ for 7 days

The qPCR system prepared with 14321 and common Taq DNA Polymerase, pre-mix the HPV target three types and internal control primers and probes with all reagent components, and place them at 37°C for 7 days. Simultaneously, test the reagents stored at -20°C with a template concentration of 10 copies/µL. The results show that the Ct value of common Taq DNA Polymerase remains basically unchanged after thermal acceleration treatment, but the fluorescence value decreases to varying degrees. In contrast, the 14321 qPCR system shows no changes in the amplification curve peak shape, fluorescence value, or Ct value after being accelerated at 37°C for 7 days compared to the reagents stored at -20°C. The 14321 qPCR system demonstrates higher stability for the qPCR pre-mixed system.

Figure 2. The 14321 qPCR system demonstrates higher stability for the qPCR pre-mixed system..

Documents:

Safety Data Sheet

EN-14321-MSDS-Ver.EN20250207.pdf

Manuals

EN_14321_Manual_Ver.EN20250207.pdf