Description
Hieff™ Bst Plus DNA Polymerase is derived from Thermophilic Geobacillus sp DNA Polymerase I, lacking 5'-3' exonuclease activity. The enzyme has stronger 5'-3' DNA polymerase activity, strand displacement activity, and dUTP tolerance, which is more suitable for anti-pollution isothermal amplification reactions, such as LAMP, CPA, etc.
Features
- Thermophilic DNA polymerase with strong strand displacement activity
Applications
- Isothermal DNA amplification
Specifications
| Polymerase | Bst DNA Polymerase |
| Heat Inactivation | Incubation at 85°C for 5 min. |
| Solution Components | 10 mmol/L Tris-HCl, 50 mmol/L KCl, 0.1 mmol/L EDTA, 1 mmol/L DTT, 0.1% Triton X-100, 50% Glycerol, pH 7.5 @ 25℃. |
| Activity Definition | 1 U refers to the amount of enzyme required to incorporate 10 nmol of dNTP into the acid-insoluble precipitate in 30 min at 65°C. |
Components
| Components No. | Name | 14402ES92 (8,000 U) | 14402ES97 (40,000 U) |
| 14402-A | Hieff™ Bst Plus DNA Polymerase (40 U/μL) | 200 μL | 1 mL |
| 14402-B | 10 ×Hieff™ Bst Plus DNA Polymerase Buffer | 1 mL | 3×1 mL |
| 14402-C | 100 mmol/L MgSO4 | 1 mL | 3×1 mL |
Figures
- High Sensitivity
Figure 1: Sensitivity Test
Red: 50 copies/T, response less than 15 minutes
Figure 1: The copy numbers of 50 copies/Tweres tested. The test results showed that within 15 minutes, the results of 50 copies/T performed better, and the detection rate reached 100%.
- Stability
Figure 2.Stability testing at the same temperature
Red: 37℃ 0 day Blue: 37℃ 1 day Green: 37℃ 3 days Purple: 37℃ 5 days
0 days at 37°C as a control and stability tests were carried out for 1, 3, and 5 days at the same temperature, indicating that it can still maintain a relatively stable effect at 37°C for 5 days.
Figure 3. -20℃ repeated freeze-thaw stability test
Red: Repeat freeze-thaw 0 times
Blue: Repeat freeze-thaw 5 times
Green: Repeat freeze-thaw 10 times
- Specificity
Figure 4. 48-well NTC-specific assay
48-well NTC detection was used in the specific detection of the product, and no peak was detected, indicating that the specificity was better.
Storage
The product should be stored at -25℃ ~ -15℃ for 2 years. Please avoid repeated freeze-thaw.
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The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
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