Description
Hieff™ Bst Plus DNA Polymerase is derived from Thermophilic Geobacillus sp DNA Polymerase I, lacking 5'-3' exonuclease activity. The enzyme has stronger 5'-3' DNA polymerase activity, strand displacement activity, and dUTP tolerance, which is more suitable for anti-pollution isothermal amplification reactions, such as LAMP, CPA, etc.
Features
- Thermophilic DNA polymerase with strong strand displacement activity
Applications
- Isothermal DNA amplification
Specifications
| Polymerase | Bst DNA Polymerase |
| Heat Inactivation | Incubation at 85°C for 5 min. |
| Solution Components | 10 mmol/L Tris-HCl, 50 mmol/L KCl, 0.1 mmol/L EDTA, 1 mmol/L DTT, 0.1% Triton X-100, 50% Glycerol, pH 7.5 @ 25℃. |
| Activity Definition | 1 U refers to the amount of enzyme required to incorporate 10 nmol of dNTP into the acid-insoluble precipitate in 30 min at 65°C. |
Components
| Components No. | Name | 14402ES92 (8,000 U) | 14402ES97 (40,000 U) |
| 14402-A | Hieff™ Bst Plus DNA Polymerase (40 U/μL) | 200 μL | 1 mL |
| 14402-B | 10 ×Hieff™ Bst Plus DNA Polymerase Buffer | 1 mL | 3×1 mL |
| 14402-C | 100 mmol/L MgSO4 | 1 mL | 3×1 mL |
Figures
- High Sensitivity
Figure 1: Sensitivity Test
Red: 50 copies/T, response less than 15 minutes
Figure 1: The copy numbers of 50 copies/Tweres tested. The test results showed that within 15 minutes, the results of 50 copies/T performed better, and the detection rate reached 100%.
- Stability
Figure 2.Stability testing at the same temperature
Red: 37℃ 0 day Blue: 37℃ 1 day Green: 37℃ 3 days Purple: 37℃ 5 days
0 days at 37°C as a control and stability tests were carried out for 1, 3, and 5 days at the same temperature, indicating that it can still maintain a relatively stable effect at 37°C for 5 days.
Figure 3. -20℃ repeated freeze-thaw stability test
Red: Repeat freeze-thaw 0 times
Blue: Repeat freeze-thaw 5 times
Green: Repeat freeze-thaw 10 times
- Specificity
Figure 4. 48-well NTC-specific assay
48-well NTC detection was used in the specific detection of the product, and no peak was detected, indicating that the specificity was better.
Storage
The product should be stored at -25℃ ~ -15℃ for 2 years. Please avoid repeated freeze-thaw.