Description
Hieff™ Super Bst DNA Polymerase is derived from Thermophilic Geobacillus sp DNA Polymerase I and has been genetically modified to remove its 5’-3’ exonuclease activity. This product possesses strong 5’-3’DNA polymerase activity, strand displacement activity, and dUTP tolerance, making it more suitable for contamination prevention in isothermal amplification reactions such as LAMP, CPA, etc. Compared to Bst Plus DNA Polymerase, Hieff™ Super Bst DNA Polymerase has improved the thermal stability of the Bst enzyme, which helps to enhance the amplification performance of the Bst enzyme. This enzyme is suitable for low-template and high-temperature LAMP reaction systems, effectively improving the specificity of detection and reducing the detection time. The high-temperature resistance characteristic is also applicable for the development of heat-resistant Bst, further enhancing the specificity of detection.
Features
Thermophilic DNA polymerase with strong strand displacement activity
Applications
LAMP, CPA, RCA etc.
High Temperature LAMP/RT-LAMP, Over 70℃
Specifications
|
Polymerase |
Bst DNA Polymerase |
|
Activity Definition |
1 U refers to the amount of enzyme required to incorporate 10 nmol of dNTP into the acid-insoluble precipitate in 30 min at 65°C. |
|
Solution Components |
10 mmol/L Tris-HCl, 50 mmol/L KCl, 0.1 mmol/L EDTA, 1 mmol/L DTT, 0.1% Triton X-100, 50% Glycerol, pH 7.5 @ 25℃. |
Components
|
Name |
14410ES10 20 KU |
14410ES50 100 KU |
14410ES60 200 KU |
|
|
14410 |
Hieff ™ Super Bst DNA Polymerase(2,000 U/μL) |
10 μL |
50 μL |
100 μL |
Figure
Figure 1. RT-LAMP testing with Hieff Super BST at 70℃.
Shipping and Storage
The product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for 2 years. Please avoid repeated freeze-thaw.
Payment & Security
Your payment information is processed securely. We do not store credit card details nor have access to your credit card information.
Inquiry
You may also like
FAQ
The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
Certain applications may require additional third-party intellectual property rights.
Yeasen is dedicated to ethical science, believing our research should address critical questions while ensuring safety and ethical standards.