Description
UDG (uracil DNA glycosylase) can catalyze the hydrolysis of the N-glycosidic link between the uracil base and the sugar-phosphate backbone in ssDNA and dsDNA. It can easily control aerosol pollution and is suitable for common molecular biology systems such as PCR, qPCR, RT-qPCR and LAMP.
Features
10 U of this product was detected by E.coli 16S rDNA-specific TaqMan qPCR, and the result showed that E.coli genome residue was fewer than 10 copies
No nucleic acid endonuclease, exonuclease and RNase residues
Uracil is the sole base recognized by this enzyme
Applications
Remove ssDNA and dsDNA, but they are inactive to RNA
Eliminate false positive results due to contamination of PCR amplification products.
Compatible with PCR, qPCR, RT-qPCR, etc
Specifications
| Expression Host | Recombinant E. coli with uracil DNA glycosylase gene |
| Molecular Weight | 24.8 kDa |
| Purity | ≥ 95%（SDS-PAGE） |
| Heat Inactivation | 95°C, 5~10 min |
| Unit Definition | One unit (U) is defined as the amount of enzyme that required to catalyze the hydrolysis of 1 μg dU-containing dsDNA in 30 minutes at 25°C. |
Components
| Components No. | Name | 14455ES60 (100 U) | 14455ES76 (500 U) | 14455ES96 (10,000 U) |
| 14455 | Uracil DNA Glycosylase (UDG/UNG), 1 U/μL | 100 μL | 500 μL | 10 mL |
Storage
The product should be stored at -25℃ ~ -15℃ for two years.
Figures
Figure 1．UDG enzyme (0.025 U) fully digested 360 ng of 200 bp dU -DNA
Electrophoresis results of 0.05 U, 0.025 U, 0.0125 U, 0.00625 U, 0.003125 U anti- contamination UDG enzyme with 360 ng of 200 bp dU -DNA incubated at 25°C for 30 min.