Description
Klenow Fragment is a proteolytic product of E. coli DNA Polymerase I, retaining the DNA polymerase activity and 3'→5' exonuclease activity of DNA Polymerase I, but lacking the 5'→3' exonuclease activity. It can be used for random priming DNA labeling and the synthesis of the second strand of cDNA.
Features
No residual nucleases or RNases
High amplification efficiency
Applications
DNA sequencing by the Sanger dideoxy method
Second strand cDNA synthesis
DNA labeling using random primers
Specifications
|
Source |
E. coli |
|
Molecular Weight |
68.2 kDa |
|
Concentration |
5 U/μL |
|
Unit Definition |
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C |
Components
|
Components No. |
Name |
14458ES70 |
14458ES80 |
|
14458-A |
Klenow Fragment (5 U/μL) |
40 μL |
200 μL |
|
14458-B |
10×Klenow Fragment Buffer |
1.25 mL |
1.25 mL |
Shipping and Storage
This product should be stored at -25 ~ -15℃ for 2 years.
Figures
Figure1. Detection of E. coli genomic DNA residue
Note: The residual E. coli genomic DNA in three batches of Klenow Fragment (Cat#14458) is below 1 copy (at 50 U).
Figure2. Product Stability Testing
Note: The Klenow Fragment enzyme from Yeasen (Cat#14458), along with its supplier A, was subjected to a stability test at 37°C for durations of 7 and 14 days to measure the residual enzymatic activity. The findings indicate that Yeasen's Klenow Fragment maintained its activity without any significant decline after a week at 37°C, and retained approximately 90% of its initial activity after two weeks.
Documents:
Safety Data Sheet
Manuals
14458_Manual_Ver.EN20241210.pdf
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