Klenow Fragment(3'→5' exo-) (5 U/μL) _ 14460ES

Klenow (3'→5'exo-) DNA polymerase is a proteolytic product of E. coli DNA polymerase I, retaining the DNA polymerase activity of DNA polymerase I but losing the 5'→3' exonuclease activity. This enzyme has further removed the 3'→5' exonuclease activity through mutation. It can be used for DNA labeling with random priming and DNA sequencing by the dideoxy method, among other applications.

Features

No nickase or exonuclease residue

Low residual host DNA

High amplification efficiency

Applications

Random primer labeling

Sanger dideoxy DNA sequencing

Synthesis of the second strand of cDNA

Synthesis of the second strand in mutagenesis reactions

Specifications

Components

Shipping and Storage

This product should be stored at -25 ~ -15℃ for 2 years.

Figures

 Figure1. Residual E.coli genomic DNA detection

Note:The residual E. coli genomic DNA in three batches of Klenow Fragment (3'→5' exo-) (Cat#14460) is below 1 copy/50 U.

 Figure2. Product Stability Testing

Note: Klenow Fragment (3'→5' exo-) from Yeasen and Supplier A was stored at 37°C for 7 and 14 days to assess residual enzyme activity. The results indicate that the residual activity of Yeasen 's Klenow Fragment (3'→5' exo-) (Cat#14460) after 14 days at 37°C is notably higher than that of the Supplier A.

  Figure3. NGS library yield

Note: Using the same amount of Klenow Fragment (3'→5' exo-) (Cat#14460) from Yeasen and Supplier A for NGS library construction, the results show that the library yield with Yeasen 's Klenow Fragment (3'→5' exo-) (Cat#14460) at both 5 U and 10 U input is higher than that of Supplier A.

Documents:

Safety Data Sheet

14460_MSDS_Ver.EN20241210.pdf

Manuals

14460_Manual_Ver.EN20241210.pdf