Description
UCF.ME Uracil DNA Glycosylase derived from psychrophilic marine bacteria, it is an ultra-low residual heat-labile UDG enzyme known as UCF.METM. This product can prevent the residual activity of conventional UDG enzymes after inactivation from degrading dU-containing amplification products at room temperature. It also prevents background bacteria present in molecular enzymes from causing false positives in PCR results. Therefore, the introduction of UCF.METM ultra-low residual thermolabile UDG enzyme is crucial for accurately identifying infectious pathogens and assisting in the clinical diagnosis of infections and infectious diseases.
Features
Ultra-low residual of UCF.METM—E. coli genomic DNA residue <0.1 copies/ U;
Low nuclease residue—No residual exonuclease, nicking enzyme, or RNase;
Strong digestion capability—0.05 U/T can digest 105 copies/T dU-DNA products;
Good thermolability—Complete inactivation under any condition of 50℃ for 10 minutes, 55℃ for 5 minutes, or 95℃ for 5 minutes;
Compatible with RT-qPCR reaction systems—No impact on the detection system even at high input levels (2U/20μL reaction system).
Specifications
Components
|
Name |
14466ES60 100 U |
14466ES76 500 U |
14466ES96 10,000 U |
|
UCF.METM Uracil DNA Glycosylase (UDG/UNG), heat-labile, 1 U/μL |
100 μL |
500 μL |
10 mL |
Storage
The product should be stored at -25~-15°C for 2 years.
Figures
Figure 1. 14466ES protein purity (SDS-PAGE) detection results. Protein Purity≥95%
Figure 2. 14466ES Nuclease residue test results. No Residual Nucleases, No Residual Exonucleases, Nicking Enzymes, or RNases.
Figure 3. 14466ES E.coli genomic DNA residue test results. E.coli genomic DNA residue< 0.1 copies/ U, the residue levels were significantly lower than the conventional UDG.
Figure 4. Strong digestive capability. 0.05 U/T is sufficient to digest 10^5 copies/T of dU-DNA products. 14466ES was used to prepare qPCR mix, while the control group of qPCR mix without UDG enzyme could completely digest 105 copies/T dU-DNA products. 04
Figure 5. After 50℃, 10min; 55℃, 5min; 55℃, 10min; 95℃, 5min heat inactivation treatment, 14466ES lost digestive ability.
Figure 6. Compatible with the RT-qPCR reaction system, with no impact on the detection system even at high input levels (2U/T).
Documents:
Safety Data Sheet
EN-14466-MSDS-Ver.EN20250205.pdf
Manuals
EN_14466_Manual_Ver.EN20240426.pdf
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The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
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