Description
Exonuclease I, sourced from a genetically engineered E. coli strain expressing the Exo I gene, exhibits exonuclease activity that digests single-stranded DNA from the 3' to 5' end. It sequentially releases deoxyribonucleotide 5'-monophosphates, preserving the integrity of the 5'-terminal dinucleotides. This enzyme is predominantly utilized for the breakdown and removal of primers following PCR amplification. It remains inactive towards double-stranded DNA and DNA strands with 3' hydroxyl ends that are blocked by phosphorylation or acetylation.
Features
No residual exonuclease (double-stranded), endonuclease, or RNase
Inactivated by simply treating it at 80°C for 15 minutes
Applications
Remove single-stranded primers from the PCR reaction system before Sanger DNA sequencing or SNP analysis
Remove single-stranded primers from the nested PCR reaction system
Eliminate linear single-stranded DNA from the sample, leaving behind double-stranded DNA
Specifications
|
Source |
E. coli |
|
Molecular Weight |
55 KDa |
|
Concentration |
20 U/μL |
|
Unit Definition |
One unit is defined as the amount of enzyme required to catalyze the release of 10 nmol of acid-soluble nucleotides from single-stranded [3H]-DNA at a concentration of 0.17 mg/ml in a 50 μL reaction system containing 1X Exonuclease I reaction buffer at 37°C within 30 minutes |
Components
|
Components No. |
Name |
14535ES80 |
14535ES90 |
|
14535-A |
Exonuclease I (20 U/μL) |
250 μL |
|
|
14535-B |
10×Exonuclease I Reaction Buffer |
1 mL |
1 mL |
Shipping and Storage
This product should be stored at -25 ~ -15℃ for 2 years.
Figures
Figure1. Digestion capability of Exonuclease I on single-stranded DNA
Note: In a 20 μL reaction system, a final concentration of 15 pmol/μL single-stranded DNA substrate was added. Different amounts (0.63, 1.25, 2.5, 5, 10, 20 U) of Yeasen's and the Supplier A's Exonuclease I were used and incubated at 37°C for 15 minutes, followed by heat inactivation at 80°C for 15 minutes. Polyacrylamide gel electrophoresis was employed to detect the degradation of single-stranded DNA. The results demonstrated that Yeasen's Exonuclease I has the same ability to digest single-stranded DNA as Supplier A.
Documents:
Safety Data Sheet
Manuals
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