Description
Exonuclease I, sourced from a genetically engineered E. coli strain expressing the Exo I gene, exhibits exonuclease activity that digests single-stranded DNA from the 3' to 5' end. It sequentially releases deoxyribonucleotide 5'-monophosphates, preserving the integrity of the 5'-terminal dinucleotides. This enzyme is predominantly utilized for the breakdown and removal of primers following PCR amplification. It remains inactive towards double-stranded DNA and DNA strands with 3' hydroxyl ends that are blocked by phosphorylation or acetylation.
Features
No residual exonuclease (double-stranded), endonuclease, or RNase
Inactivated by simply treating it at 80°C for 15 minutes
Applications
Remove single-stranded primers from the PCR reaction system before Sanger DNA sequencing or SNP analysis
Remove single-stranded primers from the nested PCR reaction system
Eliminate linear single-stranded DNA from the sample, leaving behind double-stranded DNA
Specifications
|
Source |
E. coli |
|
Molecular Weight |
55 KDa |
|
Concentration |
20 U/μL |
|
Unit Definition |
One unit is defined as the amount of enzyme required to catalyze the release of 10 nmol of acid-soluble nucleotides from single-stranded [3H]-DNA at a concentration of 0.17 mg/ml in a 50 μL reaction system containing 1X Exonuclease I reaction buffer at 37°C within 30 minutes |
Components
|
Components No. |
Name |
14535ES80 |
14535ES90 |
|
14535-A |
Exonuclease I (20 U/μL) |
250 μL |
|
|
14535-B |
10×Exonuclease I Reaction Buffer |
1 mL |
1 mL |
Shipping and Storage
This product should be stored at -25 ~ -15℃ for 2 years.
Figures
Figure1. Digestion capability of Exonuclease I on single-stranded DNA
Note: In a 20 μL reaction system, a final concentration of 15 pmol/μL single-stranded DNA substrate was added. Different amounts (0.63, 1.25, 2.5, 5, 10, 20 U) of Yeasen's and the Supplier A's Exonuclease I were used and incubated at 37°C for 15 minutes, followed by heat inactivation at 80°C for 15 minutes. Polyacrylamide gel electrophoresis was employed to detect the degradation of single-stranded DNA. The results demonstrated that Yeasen's Exonuclease I has the same ability to digest single-stranded DNA as Supplier A.
Documents:
Safety Data Sheet
Manuals
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The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
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