Exonuclease I (20 U/μL) _ 14535ES

Exonuclease I, sourced from a genetically engineered E. coli strain expressing the Exo I gene, exhibits exonuclease activity that digests single-stranded DNA from the 3' to 5' end. It sequentially releases deoxyribonucleotide 5'-monophosphates, preserving the integrity of the 5'-terminal dinucleotides. This enzyme is predominantly utilized for the breakdown and removal of primers following PCR amplification. It remains inactive towards double-stranded DNA and DNA strands with 3' hydroxyl ends that are blocked by phosphorylation or acetylation.

Specifications

Components

Storage

This product should be stored at-25~-15℃ for 2 years.

Product Application

1．Remove single-stranded primers from the PCR reaction system before Sanger DNA sequencing or SNP analysis.

2．Remove single-stranded primers from the nested PCR reaction system.

3．Eliminate linear single-stranded DNA from the sample, leaving behind double-stranded DNA.

Instructions

Removal of primers or other single-stranded residuals from PCR reaction products:

1. System Preparation:

2. Reaction Conditions: Thoroughly mix and incubate at 37°C for 15-30 minutes.

3. Deactivation: Incubate at 85°C for 15 minutes.

Notes

1. These instructions apply to PCR products generated by any DNA polymerase. Purified PCR products can be directly used for DNA sequencing, but they are not recommended for direct use in subsequent cloning procedures.

2. For any other specific experiments that require the individual removal of single-stranded DNA (ssDNA), it is recommended to use the 10× Exonuclease I Reaction Buffer.

3. This product is for research use only.

4. Please wear the necessary PPE, such lab coat and gloves, to ensure your health and safety.

Documents:

Manuals

14535_Manual