Description
Endonuclease VIII is a DNA repair enzyme that possesses both N-glycosylase activity and AP-lyase activity. The N-glycosylase activity can release damaged pyrimidine bases from double-stranded DNA, creating an abasic (AP) site. The AP-lyase activity then cleaves the phosphodiester bond at the AP site, resulting in a gap with a 5' phosphate and a 3' phosphate. The damaged bases that Endonuclease VIII recognizes and excises include: urea, 5,6-dihydroxythymine, thymine glycol, 5-hydroxy-5-methoxyhydantoin, uracil glycol, 6-hydroxy-5,6-dihydrothymidine, and methylhydroxypropanone.
Features
No residual of exonucleases, endonucleases, or RNases
High-efficiency removal of damaged bases
Applications
Single-cell gel electrophoresis (Comet assay)
Alkaline elution
Alkaline unwinding
Specifications
|
Source |
E. coli |
|
Molecular Weight |
29 KDa |
|
Concentration |
10 U/μL |
|
Unit Definition |
One unit is defined as the amount of enzyme required to cleave 1 pmol of a 34 bp oligonucleotide duplex containing one AP site* in a 10 µL total reaction system with 10 pmol of fluorescently labeled oligonucleotide duplex in 1X Endonuclease VIII Reaction buffer at 37°C within 1 hour |
|
Inactivation Conditions |
Incubate at 75°C for 10 minutes |
Components
|
Components No. |
Name |
14536ES80 |
14536ES90 |
|
14536-A |
Endonuclease VIII(10 U/μL) |
500 μL |
|
|
14536-B |
10 × Endonuclease VIII Reaction Buffer |
1.5 mL |
1.5 mL |
Shipping and Storage
The products should be stored at -25℃ ~ -15℃ for 1 year.
Figures
Figure1. Base excision repair effect comparison
Note: 20 pmol of oligonucleotides were catalyzed by Endonuclease VIII from Yeasen and SupplierA, respectively, in a 20 µL reaction system with the addition of 5~40 U of Endonuclease VIII and equivalent enzyme activity of UDG (Cat#10303ES), and incubated at 37℃ for 1 hour. The results of agarose gel electrophoresis showed that the base excision effects of Yeasen 's Endonuclease VIII and Supplier A's were consistent.
Documents:
Safety Data Sheet
Manuals
Payment & Security
Your payment information is processed securely. We do not store credit card details nor have access to your credit card information.
Inquiry
You may also like
FAQ
The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
Certain applications may require additional third-party intellectual property rights.
Yeasen is dedicated to ethical science, believing our research should address critical questions while ensuring safety and ethical standards.