Description
RNase HII is a ribonuclease internal enzyme obtained from the Pyrococcus abyssi and recombinantly expressed in E. Coli. It recognizes the DNA-rN-DNA/DNA double strand, cutting at the site where ribonucleotides are incorporated into DNA. However, its activity on single-stranded RNA is very low, and it shows no cutting activity on dsDNA or ssDNA. RNase HII cuts at the site of a single ribonucleotide residue from the 5' end, producing a 5' phosphate group and a 3' hydroxyl end after cutting. This RNase HII enzyme has optimal activity at 70~75°C, is active between 50°C and 75°C, but has very low activity at room temperature. The product is highly thermostable, with almost no loss of activity after incubation at 95°C for 45 minutes, and is compatible with various PCR reaction systems.
Specifications
Cat.No. |
14541ES80/14541ES92 |
Size |
50 U / 250 U |
Components
Name |
14539ES50 |
14539ES72 |
RNase HII(2 U/μL) |
25 μL |
125 μL |
Storage
This product should be stored at-25~-15℃ for 2 years.
Product Application
- Detection by LAMP with high-sensitivity probes.
- RNase HII-dependent PCR (rhPCR).
- Removal of mismatched ribonucleotides formed during polymerase chain reaction.
- Degradation of the RNA portion of Okazaki fragments.
Notes
- This product is for research use only.
- Please operate with lab coats and disposable gloves, for your safety.
Documents:
Manuals