Description
Endonuclease IV can act on a variety of oxidative damage in DNA. The enzyme is apurinic/apyrimidinic (AP) endonuclease that will hydrolyse intact AP sites in DNA. AP sites are cleaved at the first phosphodiester bond that is 5´ to the lesion leaving a hydroxyl group at the 3´ terminus and a deoxyribose 5´-phosphate at the 5´ terminus. The enzyme also has a 3´ -diesterease activity and can release phosphoglycolaldehyde, intact deoxyribose 5-phosphate and phosphate from the 3´ end of DNA.
Applications
Single cell gel electrophoresis (Comet assay)
Alkaline elution
Alkaline unwinding
Specifications
|
Source |
E.coli |
|
Molecular Weight |
32.5 KD |
|
Concentration |
10 U/μL |
|
Unit Definition |
One unit is defined as the amount of enzyme required to cleave 1 pmol of a 34-mer oligonucleotide duplex containing a single AP site in a total reaction volume of 10 µl in 1 hour at 37°C. An AP site is created by treating 10 pmol of a 34 mer oligonucleotide duplex containing a single uracil residue with 1 unit of Uracil-DNA Glycosylase (UDG) for 2 minutes at 37°C. |
Components
|
Components No. |
Name |
14543ES80 |
14543ES90 |
|
14543-A |
Endonuclease IV(10 U/μL) |
100 μL |
500 μL |
|
14543-B |
10 x Endonuclease IV Buffer |
0.8 mL |
1.25 mL |
Shipping and Storage
This product should be stored at-25~-15℃ for 1 years.
Documents:
Safety Data Sheet
Manuals
14543_Manual _Ver.EN20250121.pdf
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