Description
Endonuclease IV can act on a variety of oxidative damage in DNA. The enzyme is apurinic/apyrimidinic (AP) endonuclease that will hydrolyse intact AP sites in DNA. AP sites are cleaved at the first phosphodiester bond that is 5´ to the lesion leaving a hydroxyl group at the 3´ terminus and a deoxyribose 5´-phosphate at the 5´ terminus. The enzyme also has a 3´ -diesterease activity and can release phosphoglycolaldehyde, intact deoxyribose 5-phosphate and phosphate from the 3´ end of DNA.
Specifications
|
Cat.No. |
14543ES80 / 14543ES90 |
|
Size |
1000 U / 5000 U |
|
Source |
E.coli |
|
Molecular Weight |
32.5 KD |
|
Unit Definition |
One unit is defined as the amount of enzyme required to cleave 1 pmol of a 34-mer oligonucleotide duplex containing a single AP site in a total reaction volume of 10 µl in 1 hour at 37°C. An AP site is created by treating 10 pmol of a 34 mer oligonucleotide duplex containing a single uracil residue with 1 unit of Uracil-DNA Glycosylase (UDG) for 2 minutes at 37°C. |
|
Storage Buffer |
10 mM Tris-HCl, 250 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.15 % Triton®X-100, 200 µg/ml BSA, 50 % Glycerol(pH 7.4 @25℃) |
|
Concentration |
10 U/uL |
|
Purity |
≥90%（SDS-PAGE） |
Components
|
Components No. |
Name |
14543ES80 |
14543ES90 |
|
14543-A |
Endonuclease IV(10 U/uL) |
100 μL |
500 μL |
|
14543-B |
10 x Endonuclease IV Buffer |
0.8 mL |
1.25 mL |
Storage
This product should be stored at-25~-15℃ for 1 years.
Product Application
1．Single cell gel electrophoresis (Comet assay).
2．Alkaline elution.
3．Alkaline unwinding.
Notes
1. This product is for research use only.
2. Please wear the necessary PPE, such lab coat and gloves, to ensure your health and safety.
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