Description
UCF.METM Thermostable RNase H, a thermostable ribonuclease endonuclease, exhibits specific activity at elevated temperatures, targeting the phosphodiester bonds within RNA sequences of RNA:DNA hybrid structures while preserving the integrity of DNA sequences. Furthermore, it does not degrade single-stranded or double-stranded RNA or DNA substrates. Despite sharing similar ribonuclease endonuclease characteristics with its E. coli counterpart, the thermostable enzyme demonstrates enhanced activity at higher temperatures, with optimal functionality exceeding 65oC. This attribute enables UCF.METM Thermostable RNase H to be utilized in high-temperature experimental settings, ensuring heightened sensitivity and minimizing nonspecific hybridization backgrounds.
Features
High activity and excellent batch-to-batch consistency.
Low host gDNA residue: <0.02 copies/U.
Free of exonuclease, nickase, and RNase residue.
Superior stability: No significant decrease in enzyme activity after 32 days at 4oC, 16 days at 25oC, or 7 days at 37oC.
Applications
rRNA removal.
Detection of mRNA capping efficiency.
Removal of mRNA poly (A) hybridized to poly (dT).
Elimination of mRNA during cDNA second-strand synthesis.
Enhancement of amplification efficiency in isothermal amplification experiments.
Specifications
|
Cat.No. |
14545ES72 / 14545ES82 |
|
Size |
250 U / 1250 U |
|
Concentration |
5 U/μL |
|
Unit Definition |
One unit is defined as the amount of enzyme required to produce 1 nmol of ribonucleotides from 40 picomoles of a fluorescently labeled 25 base pair RNA-DNA hybrid in a total reaction volume of 50 µl in 20 minutes at 50oC |
|
Reaction conditions |
≥50oC |
Components
|
Components No. |
Name |
14545ES72 |
14545ES82 |
|
14545-A |
UCF.METM Thermostable RNase H (5 U/μL) |
50 μL |
250 μL |
|
14545-B |
10×RNase H Reaction Buffer |
1 mL |
5×1 mL |
Shipping and Storage
This product should be stored at -25 ~ -15oC for 1 year.
Figures
Figure 1. Activity Detection Results of UCF.METM Thermostable RNase H.
Note: Reaction conditions: 50°C for 20 minutes; RNA:DNA substrate - 20 pmol.
Figure 2. Host gDNA Residue Results of UCF.METM Thermostable RNase H.
Figure 3. Free of exonuclease, nickase, and RNase residue.
Figure 4. Accelerated Stability Results of UCF.METM Thermostable RNase H.
Documents:
Safety Data Sheet
Manuals
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The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
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