Description
Single-Stranded DNA Binding Protein (SSB) is a protein specifically responsible for binding to single-stranded regions of DNA. It primarily binds to the single-stranded areas produced by helicase as it moves forward along the replication fork, preventing the newly formed single-stranded DNA from re-annealing to form double-stranded DNA or being degraded by nucleases. After binding to single-stranded DNA, SSB makes the helical dimers unstable, thus allowing DNA polymerase to more easily access the substrate.The PCR reaction can be optimized by adding SSB single-stranded binding protein to the reaction mixture. SSB can be added during the PCR process to keep the denatured DNA stable, or it can be used as a PCR reaction additive to protect ssDNA from being digested by nucleases. Studies have shown that SSB can improve PCR performance on complex templates by directly or indirectly acting on the polymerase, increasing strand displacement activity, and affinity for primer templates.
Applications
Enhance the extension capability of DNA polymerase
Stabilize and label the structure of single-stranded DNA
Increase the yield and specificity of PCR reactions
Boost the yield and extension capability of reverse transcription in RT-PCR reactions
Improve DNA sequencing in regions with strong secondary structures
Specifications
|
Concentration |
5 μg/μL |
|
Unit Definition |
The protein is sold as a protein mass and the pure protein has an A280 of 0.774 at 1 mg/mL in a 1 cm path length (OD280) |
Components
|
Name |
14561ES70 |
14561ES08 |
14561ES50 |
14561ES76 |
|
Single-Stranded DNA Binding Protein, SSB (5 μg/μL) |
40 μL |
1 mL |
10 mL |
100 mL |
Shipping and Storage
The products should be stored at -25℃ ~ -15℃ for 2 years.
Documents:
Safety Data Sheet
Manuals
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