Description
T4 RNA Ligase 1, also known as T4 RNA ligase, is an ATP-dependent enzyme that catalyzes the formation of a phosphodiester bond between the 5'-P terminus and the 3'-OH terminus of single-stranded RNA (ssRNA), single-stranded DNA (ssDNA), or mononucleotide molecules. T4 RNA Ligase 1 can perform ligation between ssRNA molecules (most efficiently), between ssDNA and ssRNA (with lower efficiency), and can also be used for ligation between ssDNA molecules (with very low efficiency). It is suitable for the ligation of ssRNA molecules and for the attachment of 5' adapters in miRNA library construction for NGS.
Figure 1. Mechanism of Action of T4 RNA Ligase 1
Features
Catalyzes the formation of a phosphodiester bond between the 5'-P terminus and the 3'-OH terminus of single-stranded RNA (ssRNA), single-stranded DNA (ssDNA), or mononucleotide molecules.
Applications
RNA Cloning
5' adapters in miRNA library construction for NGS
Circular RNA Preparation
Specifications
|
Unit Definition |
One unit is defined as the amount of enzyme required to convert 1 nanomole of 5 -[32P] rA16 into a phosphatase-resistant form in 30 minutes at 37oC |
|
Source |
Recombinant protein expressed by Escherichia coli is derived from the T4 bacteriophage |
|
Reaction conditions |
10×T4 RL1 Reaction Buffer: 500 mM Tris-HCl (pH 7.5 at 25oC), 100 mM MgCl2, 20 mM DTT; Optimal incubation at 37oC |
|
Inactivation conditions |
Heating at 65oC for 15 minutes or boiling for 2 minutes can inactivate T4 RNA Ligase 1. Alternatively, adding a final concentration of 50 mM EDTA pH 8.0 can terminate the reaction of T4 RNA Ligase 1 |
Components
|
Components No. |
Name |
14651ES80 |
14651ES90 |
|
14651-A |
T4 RNA Ligase 1 (10 U/μL) |
500 μL |
|
|
14651-B |
10×T4 RL1 Reaction Buffer |
200 μL |
1 mL |
Shipping and Storage
This product should be stored at -25 ~ -15oC for 2 years.
Documents:
Safety Data Sheet
Manuals
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