Description
T4 RNA Ligase 2, also known as T4 RNA ligase 2, is an ATP-dependent double-stranded RNA ligase that exhibits both inter- and intra molecular ligation activities on RNA strands. Compared to T4 RNA Ligase 1, this enzyme displays a significantly higher activity in ligating nicks in double-stranded RNA than in ligating single-stranded RNA ends. The proximity of a 5'-phosphate group and a 3'-hydroxyl group is required for ligation. Moreover, it has the unique capability to ligate between the 3´-hydroxyl group of RNA and the 5´-phosphate group of DNA within a double-stranded structure.
Features
Strong ligation activity: capable of effectively linking dsRNA substrates.
No residual nuclease, nicking enzyme, or RNase.
Applications
Connecting the cleavage site of dsRNA.
Connecting the 3´ hydroxyl group of RNA to the 5´ phosphate group of double-stranded DNA structure.
Circular RNA preparation.
Specifications
|
Unit Definition |
One unit is defined as the amount of enzyme required to ligate 0.4 µg of an equimolar mix of a 23-mer and 17-mer RNAs in a total reaction volume of 20 µl in 30 minutes at 37oC |
|
Source |
Recombinant protein expressed by E. coli is derived from the Y10A gene of T4 bacteriophage |
|
Purity |
≥95% |
|
Reaction conditions |
React at 25oC for 1 hour |
|
Inactivation conditions |
React at 80oC for 5 min |
Components
|
Components No. |
Name |
14652ES80 |
14652ES90 |
|
14652-A |
T4 RNA ligase 2 (10 U/μL) |
100 μL |
500 μL |
|
14652-B |
10×T4 RL2 Reaction Buffer |
500 μL |
1.5 mL |
Shipping and Storage
This product should be stored at -25 ~ -15oC for 1 years.
Figures
Figure 1. Verification of T4 RNA ligase 2 ligation efficiency.
Note: The 20 μL reaction system contains dsRNA ligation substrates at a final concentration of 20 μM.
Documents:
Safety Data Sheet
Manuals
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The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
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