Description
Murine RNase Inhibitor is purified from a recombinant strain of E. coli in a soluble form. It specifically inhibits the activity of RNases A, B and C through binding noncovalently in a 1:1 ratio with high affinity. This product can be compatible with Hifair™ V Reverse Transcriptase and various DNA polymerases verified by RT-PCR and RT-qPCR. Recombinant murine RNase inhibitor does not contain 2 oxidation-sensitive cysteine which are contained in human-origin RNase inhibitor. Therefore, Murine RNase inhibitor has high anti-oxidation activity and is more stable for low DTT experiments (< 1mmol/L), such as qPCR.
Compared with Murine RNase Inhibitor (40 U/µL) (Cat#10603ES), The host nucleic acid residue of UCF.ME™ Murine RNase Inhibitor (40 U/µL) is lower，which is suitable for application with more stringent requirements on background bacteria, such as pathogen detection.
Feature
Ecoli genome:＜0.001 copies/U
Surfactants free
Application
Pathgen detection reagent
Single cell RNA-seq reagent
Related Article: Ultra low DNA Molecular Enzyme
Specifications
|
Source |
Recombinant E. coli with Murine RNase Inhibitor gene |
|
Storage Buffer |
20mM Hepes, 150 mM KCL, 8 mM DTT, 50% Glycerol, pH 7.5 @25℃ |
|
Unit Definition |
The required amount of RNase Inhibitor to inhibit 50% activity of 5-ng RNase A is defined as one unit. The activity of RNase A is measured by hydrolyzing of cyclic 2', 3'-CMP to generate 3'-CMP. |
Components
|
Name |
14672ES05 |
14672ES10 |
14672ES20 |
14672ES50 |
14672ES80 |
|
|
2 KU |
10 KU |
20 KU |
40 KU |
1000 KU |
|
UCF.ME™ Murine RNase Inhibitor (40 U/µL) |
50 μL |
250 μL |
500 μL |
1 mL |
25 mL |
Storage
This product should be stored at -25~-15℃ for 1 years.
Instructions
1. Add the following components to a nuclease-free microcentrifuge tube:
|
Components |
Volume (μL) |
|
RNase-free ddH2O |
to 20 |
|
5× Hifair™ V Buffer |
4 |
|
Oligo (dT)18 (50 μM) |
1 |
|
dNTP Mix (10 mM each) |
1 |
|
UCF.ME™ Murine RNase Inhibitor (40 U/µL) |
1 |
|
Hifair™ V Reverse Transcriptase (200 U/μL) |
1 |
|
RNA template |
Total RNA:1 ng-5 μg |
2. Mix gently.
3. Incubate at 42℃ for 45 min, and inactivate the reaction by heating at 85℃ for 5 min.
4. Store at -25~-15℃.
Notes
1. The product works in a wide pH range and exhibits maximal inhibitory activity at pH 7~8.
2. Please handle the product gently to avoid inactivation.
3. This product does not inhibit RNase H.
4. For your safety and health, please wear lab coats and disposable gloves for operation.
