Description
PBCV DNA Ligase, also known as SplintR ligase, PBCV-1 DNA Ligase, or Chlorella virus DNA ligase, is an ATP-dependent DNA ligase that efficiently catalyzes the ligation reaction of two adjacent DNA strands that are complementary to a single stranded RNA. In these two adjacent DNA strands, the one providing the 3' hydroxyl group is called the acceptor DNA strand, while the one providing the 5' phosphate group is called the donor DNA strand. During this ligation process, a complementary RNA strand acts as a "splint" or "scaffold" for the two DNA strands. The activity of PBCV DNA Ligase is much higher than that of traditional T4 DNA ligase and shows a strong affinity for DNA substrates with RNA as a splint (apparent Km = 1 nM, where a smaller Km value indicates a stronger affinity). It can detect unique RNA species at the subnanomolar level in complex mixtures, making it suitable for target enrichment processes in applications such as high-sensitivity RNA detection, next-generation sequencing, and molecular diagnostics.
Features
No residual nuclease, endonuclease, RNase, or phosphatase
Applications
Detection of microRNAs and other small RNAs based on probe method
Connecting single-stranded DNA through "clamp" fixation by complementary RNA sequences
Using DNA probes to achieve the detection of specific RNA
Detection of SNP or Alternative Splicing
RASL-seq analysis
Specifications
|
Source |
The DNA Ligase gene from PBCV-1 originating from Chlorella virus expressed in E.coli |
|
Molecular Weight |
34 KD |
|
Concentration |
25 U/µL |
|
Unit Definition |
One unit is defined as the enzyme amount required to achieve at least 50% completion of the ligation of 2 pmol FAM-labeled DNA:RNA hybrid substrate within 15 min at 25℃ in 1×PBCV Ligase buffer |
|
Inactivation Conditions |
65℃, 20 min |
Components
|
Component No. |
Name |
14962ES80 |
14962ES90 |
|
14962-A |
PBCV DNA ligase (25 U/µL) |
50 µL |
200 µL |
|
14962-B |
10 x PBCV Ligase buffer |
1 mL |
1 mL |
Shipping and Storage
The products should be stored at -25℃ ~ -15℃ for 2 years.
Documents:
Safety Data Sheet
Manuals
Payment & Security
Your payment information is processed securely. We do not store credit card details nor have access to your credit card information.