Description
The LAMP Probe Assay Kit (Bst Plus HSS) is a reagent designed for LAMP amplification using DNA as a template. It utilizes the next-generation Bst DNA Polymerase combined with an optimized buffer system to achieve highly sensitive amplification with excellent specificity. The buffer in this product does not contain nucleic acid dyes, making it suitable for probe-based detection. For fluorescence-based methods, additional dye needs to be added.
Component
|
Component Number |
Component Name |
16721ES60 (100T) |
|
16721-A |
4×Bst Buffer |
600 µL |
|
16721-B |
Bst Plus HSS DNA Polymerase (8U/µL) |
100 µL |
|
|
|
|
Storage Conditions
Store at -25°C to -15°C. Shelf life: 1 year. If not used for an extended period, store protected from light.
Instructions for Use
1. Reaction System
|
Component |
Volume (µL) |
Final Concentration |
|
4×Bst Buffer |
6 |
1× |
|
Bst Plus HSS DNA Polymerase (8U/µL) |
1 |
- |
|
Primer mix* |
2.5 |
- |
|
Template |
1-10 |
- |
|
ddH2O |
up to 25 |
- |
*Primer mix: Recommended primer concentrations are 16 µM FIP/BIP, 2 µM F3/B3, and 4 µM LoopF/LoopB. The probe concentration should be optimized based on the design. Other primer set ratios may also be tested for optimal performance.
2. Reaction Conditions
Incubate at 63-65°C for 30-60 minutes, followed by enzyme inactivation at 85°C for 5 minutes.
Note
For your safety and health, please wear a lab coat and disposable gloves while handling the reagents.
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