Description
The RT-LAMP Probe Assay Kit is a reagent designed for LAMP amplification using DNA and RNA templates. It includes the next-generation Bst Plus HSS DNA Polymerase, heat-resistant Hifair™ III Reverse Transcriptase, MRI, and an optimized buffering system, allowing for rapid and efficient LAMP/RT-LAMP probe-based detection. The Bst Plus HSS DNA Polymerase employs an aptamer-based hot start mechanism that suppresses enzymatic activity at ambient temperatures, enhancing specificity and sensitivity while enabling stable reaction assembly at room temperature.
Features
- Hotstart Bst DNA Pol
- High Sensitivity
Components
|
Components No. |
Name |
16903ES60 |
|
16903-A |
4×RT-LAMP Buffer (HSS) |
600 µL |
|
16903-B |
RT-LAMP Enzyme mix (HSS) |
100 µL |
Specifications
|
Detection method |
RT-LAMP Fluorescent probe method |
|
Enzyme mix |
Bst Plus HSS DNA Polymerase; Hifair™ III Reverse Transcriptase |
|
Type of sample |
RNA |
|
Application |
Isothermal amplification reaction |
Shipping and Storage
The product should be stored at -15℃ ~ -25℃ for 1 year. Please avoid light.
Operating Instructions
|
Components |
Volume (μL) |
Final Concentration |
|
4×RT-LAMP Buffer(HSS) |
6 |
1 × |
|
RT-LAMP Enzyme mix(HSS) |
1 |
- |
|
Primer mix* |
2.5 |
- |
|
Template |
1-10 |
- |
|
ddH2O |
up to 25 |
- |
Notes:
Recommended primer set at 16 μM FIP/BIP, 2 μM F3/B3, and 4 μM LoopF/LoopB; the probe can be added at the optimal amount confirmed by the design scheme, and the ratio of other optimal primer sets can be tested.
Reaction conditions
Incubate at a constant temperature of 63-65°C for 30-60 minutes, followed by deactivation at 85°C for 5 minutes.
Notes
For your safety and health,please wear lab coats and disposable gloves for operation.
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FAQ
The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
Certain applications may require additional third-party intellectual property rights.
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