Description
The RT-LAMP Probe Assay Kit is a reagent designed for LAMP amplification using DNA and RNA templates. It includes the next-generation Bst Plus HSS DNA Polymerase, heat-resistant Hifair™ III Reverse Transcriptase, MRI, and an optimized buffering system, allowing for rapid and efficient LAMP/RT-LAMP probe-based detection. The Bst Plus HSS DNA Polymerase employs an aptamer-based hot start mechanism that suppresses enzymatic activity at ambient temperatures, enhancing specificity and sensitivity while enabling stable reaction assembly at room temperature.
Features
- Hotstart Bst DNA Pol
- High Sensitivity
Components
|
Components No. |
Name |
16903ES60 |
|
16903-A |
4×RT-LAMP Buffer (HSS) |
600 µL |
|
16903-B |
RT-LAMP Enzyme mix (HSS) |
100 µL |
Specifications
|
Detection method |
RT-LAMP Fluorescent probe method |
|
Enzyme mix |
Bst Plus HSS DNA Polymerase; Hifair™ III Reverse Transcriptase |
|
Type of sample |
RNA |
|
Application |
Isothermal amplification reaction |
Shipping and Storage
The product should be stored at -15℃ ~ -25℃ for 1 year. Please avoid light.
Operating Instructions
|
Components |
Volume (μL) |
Final Concentration |
|
4×RT-LAMP Buffer(HSS) |
6 |
1 × |
|
RT-LAMP Enzyme mix(HSS) |
1 |
- |
|
Primer mix* |
2.5 |
- |
|
Template |
1-10 |
- |
|
ddH2O |
up to 25 |
- |
Notes:
Recommended primer set at 16 μM FIP/BIP, 2 μM F3/B3, and 4 μM LoopF/LoopB; the probe can be added at the optimal amount confirmed by the design scheme, and the ratio of other optimal primer sets can be tested.
Reaction conditions
Incubate at a constant temperature of 63-65°C for 30-60 minutes, followed by deactivation at 85°C for 5 minutes.
Notes
For your safety and health,please wear lab coats and disposable gloves for operation.
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