MolPure Magnetic Universal Viral DNA/RNA Kit _ 18521ES

MolPure^TM Magnetic Universal Viral DNA / RNA Kit is suitable for extracting viral nucleic acid from whole blood or cell-free body fluid samples (such as serum, plasma, cerebrospinal fluid, nasal / pharyngeal swab, alveolar lavage fluid, etc.). This method adopts magnetic bead purification technology, without toxic phenol chloroform extraction, safe, non-toxic and fast. The resulting products can be directly used for PCR, qPCR, second-generation sequencing and other experiments. With the automatic extraction instrument of the magnetic bead method, the high-throughput extraction of nucleic acid can be realized.

Product Information

Catalog.No	18521ES48/18521ES49/18521ES97
Sizes	48T (Bottle)/48T(Plate)/96T(Plate)

Components

Bottled Version

Category	Component Number	Component name	18521ES48
Part I	18521-A	Lysis Binding Liquid	33 mL/bottle×1
	18521-B	Magnetic Bead Suspension	1.1 mL/vial×1
	18521-C	Wash Solution A	40 mL/bottle×1
	18521-D	Wash Solution B	80 mL/bottle×1
	18521-E	Elution Solution	10 mL/bottle×1
Part Ⅱ	18521-G	Protease K	1.1 mL/vial×1

Prepackaged Version

Category	Component number	Component name	18521ES49	18521ES97
Part Ⅰ	18521-a	Lysis Binding Plate	1 Plate/Box	1 Plate/Box
	18521-b	Wash Plate	1 Plate/Box	1 Plate/Box
	18521-c	Wash + Magnetic Beads Plate Plateplate	1 Plate/Box	1 Plate/Box
	18521-d	Wash Plate	1 Plate/Box	1 Plate/Box
	18521-e	Elution Plate	1 Plate/Box	1 Plate/Box
	18521-f	Magnetic Rod Set	1 Set/Box	1 Set/Box
Part Ⅱ	18521-G	Protease K	1.1 mL/vial × 1	1.1 mL/vial × 2

Storage

Part I: Store at room temperature, transport at room temperature, with a shelf life of 1 year.

Part II: For component 18521-G (Protease K), store at 2~8℃, transport at room temperature.

Notes

1．The various buffers in this kit contain guanidinium salts. For your safety and health, please wear a lab coat

and disposable gloves during operation. Handle according to standard safety precautions to avoid contact with skin, eyes, and mucous membranes. In case of contact, immediately rinse thoroughly with water and seek medical attention.

2．If precipitation occurs in the solution, it must be heated in a 30°C water bath until the precipitate is

completely dissolved before use.

3．If the magnetic bead suspension is frozen, do not use it.

4．The various buffers in this kit contain guanidinium salts. Do not treat with oxidizing disinfectants such

as sodium hypochlorite, as this can release toxic gases. They must be handled as medical waste.

5．During elution, there may be residual magnetic beads. When aspirating samples, try to avoid inhaling the

magnetic beads.

Instructions

1. Applicable specimen types: whole blood, serum, plasma, cerebrospinal fluid, nasal/pharyngeal swabs,     

bronchoalveolar lavage fluid, and other samples.

2. Specimen storage and transportation: Specimens can be used immediately for testing or stored at -70°C or

lower for future testing with a shelf life of 6 months. Avoid repeated freezing and thawing. Specimens should be transported using cold chain logistics.

3. Freezing and thawing requirements: Quick freeze and quick thaw to avoid repeated cycles.

Operation Steps

The solution was checked for precipitation and whether the magnetic beads could be resuspended before the experiments.

Manual Extraction

1. Transfer 200-300μL of the sample into a 1.5 mL centrifuge tube, add 600 μL of lysis binding liquid,

20μL of protease K, and 20μL of magnetic bead suspension, then vortex mix for 30 seconds at high speed. Incubate at 50°C with shaking for 5-7 minutes; if the incubator does not have a shaking function, vortex three times during incubation, each for 15 seconds.

 

2. Transfer to a 1.5 mL magnetic stand for magnetic separation until the solution is clear and transparent, then aspirate and discard the solution.

3. Add 700μL of wash solution A, vortex for 10 seconds to disperse the magnetic beads, then transfer to the magnetic stand for magnetic separation until the solution is clear, and aspirate and discard the solution.

4. Add 700μL of wash solution B, vortex for 10 seconds to disperse the magnetic beads, then transfer to the magnetic stand for magnetic separation until the solution is clear, and aspirate and discard the solution.

5. Repeat step 4 with another 700 μL of wash solution B.

6. Briefly centrifuge to collect droplets on the tube wall, transfer to the magnetic stand until clear, aspirate and discard the residual liquid. Air-dry for 3-5 minutes.

Note: Ethanol residue can inhibit subsequent enzymatic reactions, so ensure ethanol evaporates completely during drying. Do not dry for too long to avoid affecting subsequent elution.

7. Add 50-100 μL of elution liquid, vortex at high speed for 2-3 minutes to disperse the magnetic beads. Incubate at 60°C for 5 minutes, then vortex at high speed for 60 seconds.

8. Briefly centrifuge to collect droplets on the tube cap into the tube, transfer to the magnetic stand until the magnetic beads are fully adsorbed, then carefully transfer the liquid to a new centrifuge tube to obtain the nucleic acid solution.

9. The nucleic acid solution can be stored at -20°C for short-term or -80°C for long-term.

 

Automated Extraction

Yeasen AP-96N automatic nucleic acid extraction and purification instrument (for more types of instruments, please contact technical support)

1. Before the experiment, pre-pack the deep hole plate with force oscillation several times to avoid liquid residue on the sealing membrane. The solution was checked for precipitation and whether the magnetic beads could be resuspended.

2. Place each 96-well plate into the nucleic acid extraction instrument in order, and place the 96-deep hole magnetic rod sleeve.

Position 1: cleave the binding plate

Position 2: rinsing board

Position 3: washing + magnetic bead plate

Position 4: washing plate

Position 6: elute plate

3. Add 200-300 µL of the sample and 20 µL of protease K to the lysis binding plate wells.

4. Run the following procedure. After the procedure, transfer the eluate from the elution plate to a new centrifuge tube. The solution can be placed in-20℃ for short-term storage and-80℃ for long-term storage.

 

AP-96N Channel Nucleic Acid Extraction Instrument Program