Description
The MolPure™ Endo-free Plasmid Maxi Kit is suitable for the rapid extraction of 0.5-2 mg of pure high-copy plasmid DNA from 150-300 mL of E. coli LB culture, with an extraction rate of up to 80-90%, featuring efficiency, fast, and convenience. The silica-based material used in the centrifugation adsorption column is a proprietary new material from our company, combined with our unique lysis buffer formulation to maximize the recovery of high-purity DNA. The extracted DNA has high purity, with extremely low endotoxin residue (<0.1 EU/μg DNA), excellent cell transfection efficiency, and can be directly used for various molecular biology experiments such as restriction digestion, transformation, PCR, in vitro transcription, and sequencing.
Features
1. The endotoxin level of the purified DNA is lower than 0.1 EU/µg.
2. The purification process is fast and can remove endotoxins simultaneously.
3. High - yield and high - copy plasmid DNA can be obtained.
4. It is suitable for the rapid extraction of 0.5-2 mg of pure high-copy plasmid DNA from 150-300 mL of E. coli LB culture
Applications
1. Gene therapy research
2. Gene silencing
3. Microinjection
4. Transfection, including primary, sensitive, and suspension cells
Specifications
|
Cat.No. |
19036ES10 |
|
Size |
10 T |
Components
|
Category |
Components No. |
Name |
19036ES10 |
|
Part I |
19036-A |
RNase A (10 mg/mL) |
750 µL |
|
19036-B |
Endotoxin-free buffer ER |
25 mL |
|
|
Part II |
19036-C |
RS Buffer E1* |
77 mL |
|
Part III |
19036-D |
MolPure DNA Column E1 |
10 pieces |
|
19036-E |
50 mL Collection Tube |
20 pieces |
|
|
19036-F |
Lysis buffer LB |
77 mL |
|
|
19036-G |
Binding buffer BD |
77 mL |
|
|
19036-H |
Protein Removal buffer PL* |
63 mL |
|
|
19036-I |
Washing buffer * |
50 mL |
|
|
19036-J |
Elution buffer |
20 mL |
Shipping and Storage
Part I components should be stored at -25~-15℃, valid for 1 year.
Part II components should be stored at 2~8℃, valid for 1 year.
Part III components should be stored at room temperature, valid for 1 year.
Figures
Documents:
Safety Data Sheet
Manuals
19036_Manual_Ver. EN20250205_EN.pdf
Citations & References:
1. Yang Y, Liu Q, Wang M, Li L, Yu Y, Pan M, Hu D, Chu B, Qu Y, Qian Z. Genetically programmable cell membrane-camouflaged nanoparticles for targeted combination therapy of colorectal cancer. Signal Transduct Target Ther. 2024 Jun 12;9(1):158. doi: 10.1038/s41392-024-01859-4. PMID: 38862461; PMCID: PMC11167040.(IF=39)
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The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
Certain applications may require additional third-party intellectual property rights.
Yeasen is dedicated to ethical science, believing our research should address critical questions while ensuring safety and ethical standards.