Description
This product consists of 10 highly purified and prestained proteins ranging in molecular weight from 8 kDa to 180 kDa (8,17,25,33,43,55,72,100,130,180kDa), among them, 72 kDa is orange band, 8 kDa is green band. The labeled apparent molecular weight was calibrated by the molecular weight Marker of standard non-prestained proteins. Using this product, the state of protein electrophoresis and the effect of membrane transfer can be dynamically observed. After SDS-PAGE electrophoresis, the color bands were transferred to PVDF membrane and NC membrane. This product is conveniently packaged and is a ready-to-use product, do not heat, dilute or add reducing agents!
After the prestained protein is combined with the dye, the molecular weight will change in the gel of different concentrations. There are signs in the instructions, this product is only for reference when judging the molecular weight of the target protein.
Features
- Bright color - three color predye, each strip average concentration of 2.5 μg
- Lower amount of this product into the gel hole - mini-gel: 3-5 μL;
- Stable performance - 50℃ for 20 h, no degradation
- Long shelf life - can be stored at -15℃ ~ -25℃ for two years
Applications
- Protein migration is monitored during SDS-PAGE electrophoresis
- Monitor protein transfer to the membrane after Western blotting
- Protein size analysis on SDS-PAGE and Western blotting
Specifications
| Product Type Specs | Protein Ladder |
| Size Range | 8 kDa to 180 kDa |
| Number of Markers | 10 |
| Molecular Weight | 8,17,25,33, 43,55,72,100,130,180 kDa |
| Stain Type | 72 kDa is orange band, 8 kDa is green band, the rest are blue bands |
| Storage Buffer | 62.5 mM Tris-H3PO4(pH 7.5), 2 mM EDTA, 2% (W/V) SDS, 33% (W/V) Glycerol, 5 mM DTT, 0.02% (V/V) proclin300 |
| Ready to Load | ready-to-use product, do not heat, dilute or add reducing agents |
Components
| Components No. | Name | 20350ES72 | 20350ES76 | 20350ES90 |
| 20350 | GoldBand™ Plus 3-color Regular Range Protein Marker (8-180 kDa) | 250 μL | 2×250 μL | 10×250 μL |
Storage
The product should be stored at -25℃ ~ -15℃ for two years. For regular use, it can be placed at 4℃ for short time use. It is recommended to store in aliquots to avoid repeated freezing and thawing!
Figures
Figure 1 12% SDS-PAGE electrophoresis results
Figure 2 Under different electrophoresis buffer conditions, the position each band of this product
[1] Nouri Z, Zhang XY, Khakisahneh S, Degen AA, Wang DH. The microbiota-gut-kidney axis mediates host osmoregulation in a small desert mammal. NPJ Biofilms Microbiomes. 2022;8(1):16. Published 2022 Apr 4. doi:10.1038/s41522-022-00280-5(IF:7.290)