Description
PNGase F is an amidohydrolase that can cleave high-mannose, hybrid, and complex oligosaccharides linked by asparagine, specifically removing N-linked glycans. However, the conventional PNGase F enzymatic reaction often takes several hours to release antibody N-glycans. Due to the preferential release of glycans, incomplete deglycosylation might result in biased outcomes, with the obtained glycan distribution failing to represent the true composition of therapeutic antibodies. Therefore, rapidly obtaining an accurate N-glycan profile is crucial for glycosylation monitoring during the production of antibodies and antibody-fusion proteins used in biotherapeutics.
Yeasen Fast PNGase F（Glycerol-free）is an optimized recombinant reagent capable of thoroughly and rapidly deglycosylating antibodies, immunoglobulins, fusion proteins, and other glycoproteins within minutes. This enzyme can swiftly and non-selectively remove all N-glycans, enabling direct downstream chromatography or mass spectrometry analysis. Yeasen Fast PNGase F（Glycerol-free） simplifies the experimental procedure while reducing experimental time without compromising sensitivity and reproducibility.
Feature
Fast—Complete and rapid deglycosylation in a few minutes.
High purity—No protease, glycosidase contamination, purity ≥95%.
Non-selective—Quickly and without preference remove all N-glycans.
Good compatibility—Directly used for downstream chromatography or MS spectrometry analysis.
Application
Glycan Sequencing
Glycoprotein Analysis
Recombinant Glycoprotein Expression
Specification
|
English synonym |
Fast PNGase F（Glycerol-free） |
|
Source |
Yeast recombinant expression |
|
Storage buffer |
20 mM Tris-HCl pH 7.5, 50 mM NaCl, 5 mM EDTA |
Components
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Components No. |
Name |
20406ES20 |
20406ES50 |
|
20406-A |
Fast PNGase F |
20 μL |
50 μL |
|
20406-B |
Fast PNGase F Buffer（5×） |
80 μL |
200 μL |
Storage
The product can be stored at 2 ~ 8℃ for one year, do not freeze.
Figures
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Figure 1. Two-step enzymatic cleavage effect (protein substrate) 1: Protein Marker(Cat#20350ES) 2: Competitor + substrate 3: Competitor + substrate 4: Yeasen Fast PNGase F + substrate 5: Yeasen Fast PNGase + substrate F 6: Substrate 7: Yeasen Fast PNGase F 8: Competitor
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Figure 2. Two-step enzymatic cleavage effect (antibody) 1: Protein Marker(Cat#20350ES) 2: Competitor + antibody 3: Competitor + antibody 4: Yeasen Fast PNGase F + antibody 5: Yeasen Fast PNGase F + antibody 6: Antibody 7: Yeasen Fast PNGase F 8: Competitor
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