Description
IdeS Protease, full name Immunoglobulin G-degrading enzyme of Streptococcus pyogenes, IdeS, is a cysteine protease produced and secreted extracellularly by the human pathogen Streptococcus pyogenes. This proteinase has extremely high substrate specificity, only recognizing IgG and cleaving at a specific site in the antibody's hinge region, resulting in the hydrolysis of IgG into intact F(ab’)2 fragments and Fc fragments. IdeS can recognize human and various other animal sources of IgG, such as human, rabbit, monkey, sheep, and human-animal chimeric IgG, etc. IdeS has unique substrate selectivity and can be used as a tool enzyme for structural characterization analysis of antibody drugs or antibody-fusion protein drugs.
This product is recombinantly expressed in E. coli, with high purity and good enzymatic activity, making it a valuable tool for characterizing therapeutic antibodies, Fc fusion proteins, and antibody-drug conjugates.
Features
High Specificity — Single cleavage site: CPAPELLG/GPSVF.
Fast Reaction — 30 minutes rapid cleavage, significantly faster than papaya proteinase and pepsin.
High Stability — Each batch of product undergoes strict quality control to ensure batch-to-batch stability.
Simple Reaction Conditions — Compatible with various pH buffer solutions, no need for reducing agents or auxiliary reagents.
Applications
IdeS enzyme is used as a tool enzyme in the preparation and structural characterization analysis of antibody drugs.
IdeS enzyme can also assist in drug development in certain fields.
Specifications
|
English synonym |
IdeS Protease |
|
Source |
E.coli recombinant expression |
|
Label |
N-terminal His Tag |
|
Purity |
Purity is greater than 95% as analyzed by SDS-PAGE and HPLC |
|
Molecular Weight |
35.3 KDa |
|
Storage buffer |
50 mM sodium phosphate, 150 mM NaCl (pH 6.6), 50% glycerol |
|
Enzyme Concentration |
40 U/μL |
|
Unit Definition |
The amount of enzyme required to cleave more than 95% of 1 μg of recombinant monoclonal IgG in 30 minutes at 37°C is defined as one unit of activity. |
Components
|
Components No. |
Name |
20412ES84 |
20412ES90 |
|
20412 |
IdeS Protease |
2000 U |
5000 U |
Shipping and Storage
The products can be stored at -15℃ ~ -25℃ for one year.
Documents:
Safety Data Sheet
Manuals
Citations & References:
[1] Cheng Y, Shi D, Ye R, et al. Noninvasive evaluation of PD-L1 expression in non-small cell lung cancer by immunoPET imaging using an acylating agent-modified antibody fragment. Eur J Nucl Med Mol Imaging. 2023;50(6):1585-1596. doi:10.1007/s00259-023-06130-6(IF:9.1)
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The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
Certain applications may require additional third-party intellectual property rights.
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