Description
Endo S, is a highly specific glycosidase derived from Streptococcus pyogenes. It can cut the N-linked glycans between the core structure of the shell di-saccharides of wild-type IgG heavy chains. This product has a purity of over 95%, high activity, good stability, no glycosidase contamination, and no proteolytic activity. For easy subsequent operations, it is equipped with a histidine (His) tag, which makes it easy to remove from the reaction.
Properties
|
English synonym |
Endo S |
|
Source |
E.coli recombinant expression |
|
Purity |
Detected by SDS-PAGE, purity > 95% |
|
Storage buffer |
PBS pH 7.5 |
|
Specific activity |
8,000 U/mg |
|
Unit Definition |
The definition of a unit is that under a total reaction volume of 10 μL, at 37 °C for 1 hour, 95% of the N-linked glycans in 5 μg of natural mouse IgG can be removed. |
Components
|
Components No. |
Name |
20413ES80 |
20413ES90 |
|
20413-A |
Endo S |
1000 U |
5×1000 U |
|
20413-B |
10×Buffer |
1 mL |
5×1 mL |
Storage
The product can be stored at -15~-25℃ for one year.
Instructions
Reaction systerm
|
Components |
Volume |
|
IgG |
V |
|
Endo S |
1 |
|
10×Buffer |
1 |
|
H2O |
to 10 |
Note: After incubation at 37 °C for 1 hour, electrophoresis detection, load 2-3 μg of samples. The control group is the original protein.
Experimental Example
Electrophoresis of IgG under Endo S enzyme conditions
Lane -: Control, without Endo S enzyme; Lane +: With Endo S enzyme.
Notes
1. Please wear the necessary PPE, such lab coat and gloves, to ensure your health and safety！
2. For research use only!
Ver. EN20240426
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