Endo S _ 20413ES

SKU: 20413ES80

Size: 1000 U
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Description

Endo S, is a highly specific glycosidase derived from Streptococcus pyogenes. It can cut the N-linked glycans between the core structure of the shell di-saccharides of wild-type IgG heavy chains. This product has a purity of over 95%, high activity, good stability, no glycosidase contamination, and no proteolytic activity. For easy subsequent operations, it is equipped with a histidine (His) tag, which makes it easy to remove from the reaction.

Properties

English synonym

Endo S

Source

E.coli recombinant expression

Purity

Detected by SDS-PAGE, purity > 95%

Storage buffer

PBS pH 7.5

Specific activity

8,000 U/mg

Unit Definition

The definition of a unit is that under a total reaction volume of 10 μL, at 37 °C for 1 hour, 95% of the N-linked glycans in 5 μg of natural mouse IgG can be removed.

Components

Components No.

Name

20413ES80

20413ES90

20413-A

Endo S

1000 U

5×1000 U

20413-B

10×Buffer

1 mL

5×1 mL

Storage

The product can be stored at -15~-25℃ for one year.

Instructions

Reaction systerm

Components

Volume

IgG

V

Endo S

1

10×Buffer

1

H2O

to 10

Note: After incubation at 37 °C for 1 hour, electrophoresis detection, load 2-3 μg of samples. The control group is the original protein.

Experimental Example

Electrophoresis of IgG under Endo S enzyme conditions

Lane -: Control, without Endo S enzyme; Lane +: With Endo S enzyme.

 Notes

1. Please wear the necessary PPE, such lab coat and gloves, to ensure your health and safety

2. For research use only!

Ver. EN20240426

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