Description
Endo H is a recombinant glycosidase that can cleave the core structure of high-mannose and some hybrid oligosaccharides in N-glycoproteins, removing the N-linked high-mannose from glycoproteins. Endo H is cloned from the corrugated actinomycete (Streptomyces plicatus) and recombinantly expressed in yeast. This product is tagged with His and is commonly used for complete deglycosylation of antibodies and related proteins.
Feature
High purity—No protease, glycosidase contamination, purity ≥95%.
High Activity— 1,000,000 U/mL.
Good compatibility—Can be used denaturing or no-denaturing conditions.
Application
Glycan Sequencing
Proteomics
Glycoprotein Analysis
Recombinant Glycoprotein Expression
Specification
|
English synonym |
Endo H |
|
Source |
Yeast recombinant expression |
|
Molecular Weight |
60 kDa |
|
Specific Activity |
1,000,000 U/mL |
|
Storage buffer |
20 mM Tris-HCl pH 7.5, 50 mM NaCl, 5 mM EDTA |
|
Unit Definition |
One unit of endoglycosidase H is defined as the amount of enzyme required to hydrolyze >95% of the oligomannose from 10 μg of denatured RNase B in 1 hour at 37°C in a 10 μL reaction volume. |
Components
|
Components No. |
Name |
20414ES92 |
20414ES97 |
|
20414-A |
Endo H |
10000 U |
50000 U |
|
20414-B1 |
Buffer 1 (10×) |
100 μL |
500 μL |
|
20414-B2 |
Buffer 2 (10×) |
200 μL |
1000 μL |
Storage
The product can be stored at -25 to -15℃ for one year.
Payment & Security
Your payment information is processed securely. We do not store credit card details nor have access to your credit card information.
Inquiry
You may also like
FAQ
The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
Certain applications may require additional third-party intellectual property rights.
Yeasen is dedicated to ethical science, believing our research should address critical questions while ensuring safety and ethical standards.