Description
Endo H is a recombinant glycosidase that can cleave the core structure of high-mannose and some hybrid oligosaccharides in N-glycoproteins, removing the N-linked high-mannose from glycoproteins. Endo H is cloned from the corrugated actinomycete (Streptomyces plicatus) and recombinantly expressed in yeast. This product is tagged with His and is commonly used for complete deglycosylation of antibodies and related proteins.
Feature
High purity—No protease, glycosidase contamination, purity ≥95%.
High Activity— 1,000,000 U/mL.
Good compatibility—Can be used denaturing or no-denaturing conditions.
Application
Glycan Sequencing
Proteomics
Glycoprotein Analysis
Recombinant Glycoprotein Expression
Specification
|
English synonym |
Endo H |
|
Source |
Yeast recombinant expression |
|
Molecular Weight |
60 kDa |
|
Specific Activity |
1,000,000 U/mL |
|
Storage buffer |
20 mM Tris-HCl pH 7.5, 50 mM NaCl, 5 mM EDTA |
|
Unit Definition |
One unit of endoglycosidase H is defined as the amount of enzyme required to hydrolyze >95% of the oligomannose from 10 μg of denatured RNase B in 1 hour at 37°C in a 10 μL reaction volume. |
Components
|
Components No. |
Name |
20414ES92 |
20414ES97 |
|
20414-A |
Endo H |
10000 U |
50000 U |
|
20414-B1 |
Buffer 1 (10×) |
100 μL |
500 μL |
|
20414-B2 |
Buffer 2 (10×) |
200 μL |
1000 μL |
Storage
The product can be stored at -25 to -15℃ for one year.
