Description
The PNGase F is a recombinant enzyme expressed in yeast, which can cleave high mannoses, heterozygotes, and complex oligosaccharide proteins linked by asparagine. The cleavage site of PNGase F is the amide bond between N-acetylglucosamine (GlcNAc) and asparagic acid residue on the inner side of the glycoprotein, and the aspartyl on the enzymatic hydrolysis protein is converted to aspartic acid. This product has a His tag and is often used for complete deglycosylation of antibodies and their related proteins.
Component
|
No. |
Name |
20415ES01 |
20415ES02 |
|
20415-A |
PNGase F |
15 KU |
75 KU |
|
20415-B1 |
Buffer 1（10×） |
150 μL |
750 μL |
|
20415-B2 |
Buffer 2（10×） |
300 μL |
1500 μL |
|
20415-B3 |
10% NP-40 |
300 μL |
1500 μL |
Specifications
|
Name |
PNGase F |
|
Source |
Expressed in Yeast |
|
Molecular weight |
36 kDa |
|
Specific activity |
750000 U/mL |
|
Buffer |
20 mM Tris-HCl pH 7.5, 50 mM NaCl, 5 mM EDTA,50% Glycerol |
|
Unit Definition |
One unit is defined as the amount of enzyme required to remove > 95% of the carbohydrate from 10 µg of denatured RNase B in 1 hour at 37°C in a total reaction volume of 10 µL. |
Shipping and Storage
The products are shipped with dry ice and can be stored at -15℃ ~ -25℃ for one year.
Payment & Security
Your payment information is processed securely. We do not store credit card details nor have access to your credit card information.
Inquiry
You may also like
FAQ
The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
Certain applications may require additional third-party intellectual property rights.
Yeasen is dedicated to ethical science, believing our research should address critical questions while ensuring safety and ethical standards.