Description
Recombinant TEV protease (rTEV Protease) is a recombinant protease modified and purified by genetic engineering, which not only maintains the functional activity of natural TEV Protease, but also shows stronger stability and specificity over a broad temperature range. rTEV Protease is a commonly tool enzyme for cutting affinity labels on fusion protein. It is specific to the seven amino acid sequence EXXYXQ↓(G/S), and the cutting site is between glutamine and glycine/serine. The commonly used seven amino acid sequence is Glu-Asn-Leu-Tyr-Phe-Gln↓-Gly. rTEV Protease is optimally active at pH 7.0, 30°C, but is active over a wide range of pH 6.0-8.5 and temperatures 4-30°C (see Table 1), allowing the choice of reaction conditions to be flexible depending on the target protein. In addition, the 6×His label of N-terminal can also be used to remove rTEV Protease by Ni-NTA resin to purify the target protein.
The product has been optimized on the basis of 20403ES, and compared with 20403ES, it has higher purity, stronger activity and lower E.coli gDNA residue, which is suitable for application with higher requirements for enzyme purity, activity and residue.
Features
High cleavage efficiency: The protein tag is removed more thoroughly.
Low residual host gDNA: Effectively reduce the risk of introducing exogenous substance residues.
Applications
Removal of fusion tags
Specifications
|
Unit Definition |
The amount of enzyme required to cut >85% of the 3 μg substrate in a 1×rTEV Buffer (50 mM Tris, pH 8.0, 0.1 mM EDTA, 1 mM DTT) at 30oC for 1 h was defined as a unit of activity |
|
Protein purity |
≥95% |
|
Source |
Expressed in E. coli |
Components
|
Components No. |
Name |
20427ES80 |
20427ES10 |
20427ES50 |
|
20427-A |
rTEV Protease (5 U/μL) |
200 μL |
2×1 mL |
10 mL |
|
20427-B |
rTEV Buffer (10×) |
600 μL |
6 mL |
30 mL |
Shipping and Storage
rTEV Protease, stored at -25~-15oC for 6 months; Store at -85~-65oC for 1 year.
rTEV Buffer, stored at -25~-15oC for 1 year.
Note: It is recommended to store each component separately when receiving the goods or using it for the first time to avoid repeated freezing and thawing.
Figures
Figure 1. Verification of Cleavage Activity of UCF.METM rTEV Protease
Note: Using a fusion protein (3 μg) containing the UCF.METM rTEV Protease cleavage site as the substrate, UCF.METM rTEV Protease was added at 10, 5, and 3 U, respectively. The reaction was carried out at 30 oC for 1 hour, and the cleavage effect was detected by agarose gel electrophoresis. The results showed that when the amount of UCF.METM rTEV Protease was more than 3 U, it could cleave the substrate efficiently with a cleavage efficiency of >90%.
Documents:
Safety Data Sheet
Manuals
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