Description
Anti-DYKDDDDK (Flag) Affinity Gel is prepared by conjugating high quality murine IgG2b monoclonal antibody with 4% highly cross-linked agarose gel. This product has a high Flag label fusion protein loading capacity (at least 1.1 mg protein/mL gel) and less non-specific binding of miscellaneous proteins. It can be used for immunoprecipitation (IP) and purification of fusion proteins with Flag labels.
Anti-DYKDDDDK (FLAG) Affinity Gel can bind to the Met modified N-flag fusion Protein (MET-flag-Protein), N-flag fusion protein (Flag-protein), C-terminal FLAG Fusion Protein (protein-flag).
Features
High Specificity—The selection of high quality murine IgG2b monoclonal antibody can specifically adsorb the Flag protein sequence.
high yield and high purity—Obtain high purity Flag label protein
High binding capacity—Capacity ≥1.1mg protein /mL gel
Many types of protein binding—Met-flag-Protein, N-flag fusion protein, C-terminal FLAG Fusion Protein
Widely used—Can be used for protein purification or IP
Applications
Immunoprecipitation (IP)
Purification of fusion proteins with Flag labels.
Specifications
|
Clone |
1A3 |
|
Isotype |
Mouse IgG2b |
|
Application |
Protein purification、IP |
|
Protein binding capacity |
≥1.1 mg protein/mL Gel |
|
Storage buffer |
TBS，50% glycerin，pH7.4，contains 0.02% (w/v) sodium azide |
Components
|
Components No. |
Name |
20584ES03 |
20584ES08 |
20584ES25 |
20584ES60 |
|
20584 |
Anti-DYKDDDDK (Flag) Affinity Gel |
1 mL |
5 mL |
25 mL |
100 mL |
Note: In order to facilitate customers to use 1×Flag polypeptide elution, this product package is accompanied by 1×Flag polypeptide (Cat#20572ES).
Based on the different specifications of 1×Flag polypeptide (Cat#20572ES), 1 mL gel package is combined with 5 mg peptide, and other gel specifications are combined with 1 mL: 1 mg ratio of 5 mg specification 1×Flag polypeptide (Cat#20572ES).
Shipping and Storage
The products are shipped with ice pack, and unopened products can be stably stored at -25~-15℃ for 1 year. Do not freeze in solution without glycerin!
Figures
|
Figure 1. The SDS-PAGE gel electrophoresis results of Protein purification with Anti-Flag Affinity Gel (20584ES).
1: electrophoresis bands were purified by adding enterobacter lysate 2: negative control |
|
|
Figure 2. Purification of low expression protein by Anti-Flag affinity gel (20584ES) Affinity Gel. a：Flag-N b：Flag-M c：Flag-C M：Protein Marker 1：Flag-C pre-column stock solution 2：Flag-CPost-column penetrating solution 3: elution (pH2.7) 4: elution (pH12 ) 5: elution (1×Flag polypeptide ) 6: purified gel (pH2.7 elution) 7: purified gel (pH12 elution) 8: Purified gel (1×Flag polypeptide elution) |
Documents:
Manuals