Description
Anti-His Affinity Gel is prepared by conjugating high quality murine IgG1 monoclonal antibody with Sepharose 4B gel. This product has a high His label fusion protein loading capacity (at least 1.1 mg protein/mL gel) and less non-specific binding of miscellaneous proteins. It can be used for immunoprecipitation (IP) and purification of fusion proteins with His labels.
Features
- Specific—highly specific monoclonal antibody
- High yield and high purity
- Versatile—It can be used for protein purification or COIP
- Convenient—reagents and peptides to elute are available separately
Applications
- Immunoprecipitation (IP)
- Purification of fusion proteins with His labels.
Specifications
| Isotype | Mouse IgG1 |
| Purity | Protein A |
| Antibody Concentration | 7.5 g antibody/L Gel |
| Protein binding capacity | ≥1.1 mg protein/mL Gel |
| Storage buffer | TBS, 50% glycerin, pH7.4, contains 0.02% (w/v) sodium azide |
Components
| Components No. | Name | 20589ES03 | 20589ES08 | 20589ES25 | 20589ES60 |
| 20589 | Anti-His Affinity Gel | 1 mL | 5 mL | 25 mL | 100 mL |
Storage
The unopened products should be stably stored at -25℃ ~ -15℃ for 1 year. Do not freeze in solution without glycerin!
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